天然产物研究与开发
天然產物研究與開髮
천연산물연구여개발
NATURAL PRODUCT RESEARCH AND DEVELOPMENT
2009年
5期
727-730
,共4页
魏国柱%王浩鑫%李刚%沈月毛%赵沛基
魏國柱%王浩鑫%李剛%瀋月毛%趙沛基
위국주%왕호흠%리강%침월모%조패기
内生菌%云南美登木%烯丁酸内酯
內生菌%雲南美登木%烯丁痠內酯
내생균%운남미등목%희정산내지
endophytes%Maytenus hookeri%butenolides
从云南美登木茎皮组织分离得到内生菌Ly221,经16S rDNA部分核酸序列鉴定该菌属于链霉菌属(Streptomyces).从其液体发酵提取物中分离得到5个化合物,经波谱分析鉴定其结构分别为:4,10-dihydroxy-10-methyl-dodec-2-en-1,4-oHde(1)、两个非对映异构体4,11-dihydroxy-10-methyldodec-2-en-1,4-olides(2/3)、4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide(4)和N-acetyltyramine(5).
從雲南美登木莖皮組織分離得到內生菌Ly221,經16S rDNA部分覈痠序列鑒定該菌屬于鏈黴菌屬(Streptomyces).從其液體髮酵提取物中分離得到5箇化閤物,經波譜分析鑒定其結構分彆為:4,10-dihydroxy-10-methyl-dodec-2-en-1,4-oHde(1)、兩箇非對映異構體4,11-dihydroxy-10-methyldodec-2-en-1,4-olides(2/3)、4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide(4)和N-acetyltyramine(5).
종운남미등목경피조직분리득도내생균Ly221,경16S rDNA부분핵산서렬감정해균속우련매균속(Streptomyces).종기액체발효제취물중분리득도5개화합물,경파보분석감정기결구분별위:4,10-dihydroxy-10-methyl-dodec-2-en-1,4-oHde(1)、량개비대영이구체4,11-dihydroxy-10-methyldodec-2-en-1,4-olides(2/3)、4-hydroxy-10-methyl-11-oxo-dodec-2-en-1,4-olide(4)화N-acetyltyramine(5).
The strain Ly221 was isolated from the branch tissue of Maytenus hookeri and determined to be a member of Streptomyces according to the 16S rDNA sequence. The extracts from the fermentation broth of Streptomyces sp. Ly221 were purified,and five compounds were obtained. They were identified to be 4,10-dihydroxy-10-methyl-dodec-2-en-l ,4-olide (1) , two diastereomeric 4,11 -dihydroxy-10-methyldodec-2-en-1, 4-olides (2/3 ) , 4-hydroxy-10-methyl-l 1-oxo-do-dec-2-en-1 ,4-olide(4) and N-acetyltyramine(5) based on spectral data,respectively.
