CAJ | 학술논문

目的研究人工合成的植物雌激素-依普拉芬对去卵巢大鼠骨密度及生物力学的影响;同时与雌激素对照,探讨其骨保护效果及NO调控作用机制.方法 60只6月龄SD雌性大鼠,随机分成6组:假手术组和手术切除大鼠双侧卵巢组,后者分为阴性对照组、依普拉芬低、中、高剂量组和雌激素对照组, 分别给予基础饲料和不同剂量受试物,12周后进行骨密度、血清NO及NOS浓度测定;免疫组化方法检测股骨NOS表达.结果与假手术组相比,去卵巢大鼠阴性对照组股骨密度、生物力学指标和血清NO、eNOS浓度以及股骨eNOS表达明显降低,血清iNOS浓度以及股骨iNOS表达增加,依普拉芬组骨密度、生物力学指标血清NO、eNOS浓度以及股骨eNOS表达均高于阴性对照组,与假手术组差异无显著意义.同时低于雌激素组.血清iNOS浓度以及股骨iNOS表达均低于阴性对照组,与假手术组以及雌激素组差异无显著意义.结论一定浓度的依普拉芬可以通过提高去卵巢大鼠eNOS的表达来提高NO的浓度,促进成骨,增加骨密度,达到防治骨质疏松症的作用.
목적 연구인공합성적식물자격소-의보랍분대거란소대서골밀도급생물역학적영향;동시여자격소대조,탐토기골보호효과급NO조공작용궤제.방법 60지6월령SD자성대서,수궤분성6조:가수술조화수술절제대서쌍측란소조,후자분위음성대조조、의보랍분저、중、고제량조화자격소대조조, 분별급여기출사료화불동제량수시물,12주후진행골밀도、혈청NO급NOS농도측정;면역조화방법검측고골NOS표체.결과 여가수술조상비,거란소대서음성대조조고골밀도、생물역학지표화혈청NO、eNOS농도이급고골eNOS표체명현강저,혈청iNOS농도이급고골iNOS표체증가,의보랍분조골밀도、생물역학지표혈청NO、eNOS농도이급고골eNOS표체균고우음성대조조,여가수술조차이무현저의의.동시저우자격소조.혈청iNOS농도이급고골iNOS표체균저우음성대조조,여가수술조이급자격소조차이무현저의의.결론 일정농도적의보랍분가이통과제고거란소대서eNOS적표체래제고NO적농도,촉진성골,증가골밀도,체도방치골질소송증적작용.
Objective To evaluate the effect of Ipriflavone(7-isopropoxyisoflavone, IP)on bone mineral density(BMD) and biomechanics and synthesis of nitric oxide (NO) and nitric oxide synathase(NOS) in ovariectomized (OVX) rats. Methods 60 six-month-old Sprague-Dawley female rats were randomly assigned to six groups: sham, OVX, ovx+ipriflavone (50 mg/kg/d), ovx+ipriflavone (100 mg/kg/d) ovx+ipriflavone (200 mg/kg/d) and ovx+17β-estradiol (E2) (10ug/kg/d). Diet and different doses of the test compounds were given. After 12weeks, bone mineral density(BMD)、indexes of biomechanics and the level of serum NO and NOS were detected in grouped rats. The expression of NOS in femur was detected by immunohistochemistry and in situ hybridization. Results In comparison with sham group,BMD and the level of serum NO、 eNOS、expression of eNOS in femur of ovariectomized(OVX) rats decreased significantly(P<0.01). The level of serum iNOS and expression of iNOS in femur increased significantly(P<0.01). Some indexes of biomechanical decreased significantly(P<0.01). Ipriflavone could significantly increase BMD、the level of serum NO、eNOS、expression of eNOS in femur and some indexes of biomechanical comparison with OVX(P<0.01). There was no statistical significance of the difference between Ipriflavone and sham group. Estrogen group obtained higher level of NO and eNOS than other groups. The level of serum iNOS and expression of iNOS in femur were less than OVX group(P<0.01). There was no statistical significance of the difference betweenIpriflavone,17β-estradiol and sham group. Conclusions Ipriflavone could increase significantly the synsthesis of NO by promoting expression of eNOS in ovariectomized (OVX) rats and consequently increase BMD of OVX rats and benefit postmenopausal osteoporosis (PMOP).