植物病理学报
植物病理學報
식물병이학보
ACTA PHYTOPATHOLOGICA SINICA
2009年
5期
544-548
,共5页
刘金亮%邵云华%张广民%竺晓平%杨广玲%时呈奎%李向东
劉金亮%邵雲華%張廣民%竺曉平%楊廣玲%時呈奎%李嚮東
류금량%소운화%장엄민%축효평%양엄령%시정규%리향동
pumpkin%Zucchini yellow mosaic virus%phylogenetic analysis
Zucchini yellow mosaic virus (ZYMV) was detected by RT-PCR from pumpkin (Cucurbita mos-chata) plant showing yellowing and mosaic symptom from Liaocheng, Shandong Province. The 3'-termial 1 684 bp genomic sequence covered 633 bp of NIb encoding sequence, 840 bp of cp gene and 211 bp of 3'-untranslated region of the isolate ZYMV-Liaocheng was determined. The cp gene of ZYMV-Liaocheng shared identities of 81.4% -98.8% and 89.4% -99.5% at nucleotide and amino acid levels, respectively, with other ZYMV sequences available in the GenBank. Phylogenetic analysis indicated that ZYMV could be clus-tered to 6 genotypes. ZYMV-Liaocheng belonged to genotype I, which contained isolates from Asia, Europe and America. Genotypes Ⅲ andⅤwere unique and contained only isolates from East Asia. The isolates from East Asia had the highest variability.
