分析试验室
分析試驗室
분석시험실
ANALYTICAL LABORATORY
2010年
1期
21-24
,共4页
尚军%曹伟%魏翠梅%胡劲波
尚軍%曹偉%魏翠梅%鬍勁波
상군%조위%위취매%호경파
离子注入%辣根过氧化酶%纳米钴%ITO
離子註入%辣根過氧化酶%納米鈷%ITO
리자주입%랄근과양화매%납미고%ITO
Ion implantation%Horseradish peroxidase%Cobalt nanoparticles%ITO
辣根过氧化酶(HRP)在Co/NH_2/ITO离子注入电极上有一对良好的氧化还原峰, 峰电位分别为E_(pc)=-0.2 V, E_(pa)=-0.01 V (vs Ag/AgCl). 该修饰电极对H_2O_2具有催化作用, 可以用作H_2O_2的生物传感器, 峰电流与H_2O_2的浓度分别在1.0×10~(-10)～2.0×10~(-8) mol/L和2.0×10~(-8)～1.0×10~(-7) mol/L范围内呈线性关系, 线性回归方程分别为I_p(mA)=2.2986+0.06632 c(nmol/L)和I_p(mA)＝3.5788+7.3053E-4c(nmol/L), 相关系数分别为0.9972和0.9688. 检出限为1.0×10~(-10) mol/L.
辣根過氧化酶(HRP)在Co/NH_2/ITO離子註入電極上有一對良好的氧化還原峰, 峰電位分彆為E_(pc)=-0.2 V, E_(pa)=-0.01 V (vs Ag/AgCl). 該脩飾電極對H_2O_2具有催化作用, 可以用作H_2O_2的生物傳感器, 峰電流與H_2O_2的濃度分彆在1.0×10~(-10)～2.0×10~(-8) mol/L和2.0×10~(-8)～1.0×10~(-7) mol/L範圍內呈線性關繫, 線性迴歸方程分彆為I_p(mA)=2.2986+0.06632 c(nmol/L)和I_p(mA)＝3.5788+7.3053E-4c(nmol/L), 相關繫數分彆為0.9972和0.9688. 檢齣限為1.0×10~(-10) mol/L.
랄근과양화매(HRP)재Co/NH_2/ITO리자주입전겁상유일대량호적양화환원봉, 봉전위분별위E_(pc)=-0.2 V, E_(pa)=-0.01 V (vs Ag/AgCl). 해수식전겁대H_2O_2구유최화작용, 가이용작H_2O_2적생물전감기, 봉전류여H_2O_2적농도분별재1.0×10~(-10)～2.0×10~(-8) mol/L화2.0×10~(-8)～1.0×10~(-7) mol/L범위내정선성관계, 선성회귀방정분별위I_p(mA)=2.2986+0.06632 c(nmol/L)화I_p(mA)＝3.5788+7.3053E-4c(nmol/L), 상관계수분별위0.9972화0.9688. 검출한위1.0×10~(-10) mol/L.
With Co/NH_2/ITO ion implantation modified electrode as the working electrode, a sensitive redox peak of HRP was obtained by cyclic voltammetry. The peak potentials were -0.2 V and -0.01 V (vs Ag/AgCl). The electrocatalytic reduction of H_2O_2 was tested by cyclic voltammetry. The linear ranges of H_2O_2 at the HRP/Co/NH_2/ITO electrode were 1.0×10~(-10)～2.0×10~(-8) mol/L and 2.0×10~(-8)～1.0×10~(-7) mol/L, respectively. The detection limit of H_2O_2 at the HRP/Co/NH_2/ITO electrode is 1.0×10~(-10) mol/L.
