CAJ | 학술논문

目的克隆天花粉蛋白(trichosanthin,TCS)基因及研究重组TCS诱发大肠癌细胞LoVo凋亡的作用.方法 RT-PCR法克隆TCS基因的cDNA序列并表达纯化TCS;MTT法测定TCS对LoVo细胞生长抑制作用,评估TCS体外诱导LoVo凋亡的作用;琼脂糖凝胶电泳法进行细胞凋亡DNA分析.结果成功克隆了TCS基因,TCS对LoVo细胞体外生长具有抑制作用, DNA电泳呈梯状条带,表明TCS对LoVo细胞确实存在诱导凋亡作用,TCS对LoVo细胞半数抑制浓度(IC50)为45.0 μg/mL.结论 TCS体外能抑制LoVo细胞生长并诱导细胞凋亡,为今后TCS应用于大肠癌的临床治疗提供了重要的实验依据.
목적 극륭천화분단백(trichosanthin,TCS)기인급연구중조TCS유발대장암세포LoVo조망적작용.방법 RT-PCR법극륭TCS기인적cDNA서렬병표체순화TCS;MTT법측정TCS대LoVo세포생장억제작용,평고TCS체외유도LoVo조망적작용;경지당응효전영법진행세포조망DNA분석.결과 성공극륭료TCS기인,TCS대LoVo세포체외생장구유억제작용, DNA전영정제상조대,표명TCS대LoVo세포학실존재유도조망작용,TCS대LoVo세포반수억제농도(IC50)위45.0 μg/mL.결론 TCS체외능억제LoVo세포생장병유도세포조망,위금후TCS응용우대장암적림상치료제공료중요적실험의거.
Objective To clone cDNA of trichosanthin (TCS) and purify TCS, and to study its influence on apoptosis and growth inhibition of colorectal carcinoma LoVo cells in vitro. Methods MTT assay was adopted to measure the growth inhibition ratio of LoVo cells treated with TCS, and apoptosis was assayed by agarose gel eletrophoresis. Results The results showed that the higher concentration of TCS and the longer testing time, the stronger growth inhibition of LoVo cells. DNA agarose gel eletrophoresis showed a gradient, which confirmed that TCS could induce the apoptosis of LoVo cells. Conclusions TCS can inhibit the growth of LoVo cells in vitro and induce its apoptosis. Our study provides evidence for the application of TCS in clinical treatment of human colorectal carcinoma.