中国法医学杂志
中國法醫學雜誌
중국법의학잡지
CHINESE JOURNAL OF FORENSIC MEDICINE
2010年
2期
84-86
,共3页
张蕾萍%杜鸿雁%栾玉静%于忠山%常静%何毅%崔巍
張蕾萍%杜鴻雁%欒玉靜%于忠山%常靜%何毅%崔巍
장뢰평%두홍안%란옥정%우충산%상정%하의%최외
法医毒物分析%褪黑素%固相萃取%气相色谱
法醫毒物分析%褪黑素%固相萃取%氣相色譜
법의독물분석%퇴흑소%고상췌취%기상색보
forensic toxicological analysis%melatonin%solid phase extraction%gas chromatography
目的 建立血样中褪黑素的同相萃取方法 .方法 采用Oasis HLB固相萃取柱提取,GC/NPD定量检验,GC/MS定性检验.结果 血样中褪黑素平均萃取回收率达80%以上,最低检出限0.02μg/mL,线性良好,相关系数R~2=0.9978.结论 该方法 操作简便快速,提取回收率高,重现性好,提取物干净,可用于实际案件当中.
目的 建立血樣中褪黑素的同相萃取方法 .方法 採用Oasis HLB固相萃取柱提取,GC/NPD定量檢驗,GC/MS定性檢驗.結果 血樣中褪黑素平均萃取迴收率達80%以上,最低檢齣限0.02μg/mL,線性良好,相關繫數R~2=0.9978.結論 該方法 操作簡便快速,提取迴收率高,重現性好,提取物榦淨,可用于實際案件噹中.
목적 건립혈양중퇴흑소적동상췌취방법 .방법 채용Oasis HLB고상췌취주제취,GC/NPD정량검험,GC/MS정성검험.결과 혈양중퇴흑소평균췌취회수솔체80%이상,최저검출한0.02μg/mL,선성량호,상관계수R~2=0.9978.결론 해방법 조작간편쾌속,제취회수솔고,중현성호,제취물간정,가용우실제안건당중.
Objective To develop a solid phase extraction method for determination melatonin in human blood. Methods Purifying by using Oasis HLB solid phase extraction column, quantitatively detecting with gas chromatography (GC/NPD)and qualitatively analyzing by gas chromatography-mass spectrometry (GC-MS). Results The mean recovery rate of melatonin in human blood is over 80%, the limits of detection is 0. 02μg/mL. The method showed excellent linearity (the linear correlation coefficient is 0.9978). Conclusion It can be concluded that the method may be widely used in forensic toxicological analyses, due to its simple and quick operation, high recovery rate, good repetition, and clean extraction.
