中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2010年
7期
910-914
,共5页
呼吸道合胞体病毒感染/代谢/免疫学%GATA3转录因子/代谢%白细胞介素4/代谢%白细胞介素5/代谢
呼吸道閤胞體病毒感染/代謝/免疫學%GATA3轉錄因子/代謝%白細胞介素4/代謝%白細胞介素5/代謝
호흡도합포체병독감염/대사/면역학%GATA3전록인자/대사%백세포개소4/대사%백세포개소5/대사
Respiratory syncytial virus infections/ME/IM%GATA3 transcription factor/ME%Interleukin-4/ME%Interleukin-5/ME
目的 探讨呼吸道合胞病毒(RSV)感染时肺GATA-3表达对Th2类细胞因子分泌的调控作用.方法 将20只SD大鼠按数字表法分为对照组、RSV感染组(RSV滴鼻法建立RSV感染模型),每组10只.在第7周测定气道反应性;取肺组织苏木素伊红(HE)染色观察病理改变;通过蛋白质印迹法(western-blots)检测GATA-3在肺组织中的表达;酶联免疫吸附试验(ELISA)检测肺组织匀浆白细胞介素4(IL-4)和白细胞介素5(IL-5)水平.结果 RSV感染组大鼠肺部病理学切片呈现典型的间质性肺炎表现,而且RSV感染组气道阻力增高程度明显高于对照组大鼠.RSV感染组肺组织中GATA-3/GAPDH相对灰度值(1.743±0.21)高于对照组(1.231±0.31)(t=15.43,P＜0.01).RSV感染组IL-4[(142.3±12.3)ng/L]及IL-5蛋白水平[(167.5±14.2)ng/L]均高于对照组[(69.2±11.6)ng/L,(86.6±14.3)ng/L](P＜0.01).肺GATA-3蛋白水平与IL-4蛋白及IL-5蛋白水平呈正相关(r=0.864,0.756,P＜0.01).结论 RSV感染可以上调GATA-3在肺组织中的表达,并与Th2型炎症反应呈正相关.
目的 探討呼吸道閤胞病毒(RSV)感染時肺GATA-3錶達對Th2類細胞因子分泌的調控作用.方法 將20隻SD大鼠按數字錶法分為對照組、RSV感染組(RSV滴鼻法建立RSV感染模型),每組10隻.在第7週測定氣道反應性;取肺組織囌木素伊紅(HE)染色觀察病理改變;通過蛋白質印跡法(western-blots)檢測GATA-3在肺組織中的錶達;酶聯免疫吸附試驗(ELISA)檢測肺組織勻漿白細胞介素4(IL-4)和白細胞介素5(IL-5)水平.結果 RSV感染組大鼠肺部病理學切片呈現典型的間質性肺炎錶現,而且RSV感染組氣道阻力增高程度明顯高于對照組大鼠.RSV感染組肺組織中GATA-3/GAPDH相對灰度值(1.743±0.21)高于對照組(1.231±0.31)(t=15.43,P＜0.01).RSV感染組IL-4[(142.3±12.3)ng/L]及IL-5蛋白水平[(167.5±14.2)ng/L]均高于對照組[(69.2±11.6)ng/L,(86.6±14.3)ng/L](P＜0.01).肺GATA-3蛋白水平與IL-4蛋白及IL-5蛋白水平呈正相關(r=0.864,0.756,P＜0.01).結論 RSV感染可以上調GATA-3在肺組織中的錶達,併與Th2型炎癥反應呈正相關.
목적 탐토호흡도합포병독(RSV)감염시폐GATA-3표체대Th2류세포인자분비적조공작용.방법 장20지SD대서안수자표법분위대조조、RSV감염조(RSV적비법건립RSV감염모형),매조10지.재제7주측정기도반응성;취폐조직소목소이홍(HE)염색관찰병리개변;통과단백질인적법(western-blots)검측GATA-3재폐조직중적표체;매련면역흡부시험(ELISA)검측폐조직균장백세포개소4(IL-4)화백세포개소5(IL-5)수평.결과 RSV감염조대서폐부병이학절편정현전형적간질성폐염표현,이차RSV감염조기도조력증고정도명현고우대조조대서.RSV감염조폐조직중GATA-3/GAPDH상대회도치(1.743±0.21)고우대조조(1.231±0.31)(t=15.43,P＜0.01).RSV감염조IL-4[(142.3±12.3)ng/L]급IL-5단백수평[(167.5±14.2)ng/L]균고우대조조[(69.2±11.6)ng/L,(86.6±14.3)ng/L](P＜0.01).폐GATA-3단백수평여IL-4단백급IL-5단백수평정정상관(r=0.864,0.756,P＜0.01).결론 RSV감염가이상조GATA-3재폐조직중적표체,병여Th2형염증반응정정상관.
Objective To study the expression regulation of GATA-3 on Th2 cytokines secretions in rats lung infected by respiratory syncytial virus (RSV).Methods 20 SD rates were random divided into two groups: Control group (10 rats) and RSV-infected group(10 rats, the model was established by RSV intravasation).The airway responses were observed after seven week.Lung tissue of SD rats were stained with HE, pathologic examinations of lung tissue- stained were made.GATA-3 expressions in lung tissue were detected by western-blots, respectively.Interleukin-4 (IL-4) and interleukin-5 (IL-5) in lung homogenate were detected by ELISA.Results Lung biopsy from RSV-infected group showed typical inflammation under light microscope.Airway resistance in RSV- infected group was higher than control group.Compared with control group( 1.231 ±0.31 ), the expression of GATA-3 significantly increased in RSV-infected group (1.743 ±0.21)( t =15.427, P ＜0.01).The levels of IL-4[(142.3 ± 12.3)ng/L] and IL-5[(167.5 ±14.2) ng/L] increased in RSV-infected group [(69.2±11.6) ng/L, ( 86.6 ± 14.3 ) ng/L] ( P＜0.01 ).There was a positive correlation between protein levels of GATA-3 and IL-4 in lung tissues (r=0.864, P＜0.01 ), and a positive correlation between GATA-3 and IL-5 in lung tissues(r=-0.756, P＜0.01).Conclusion RSV-infection may up regulate the expression of GATA-3 in lung, which contributed to inflammation of Th2 type.
