中华核医学杂志
中華覈醫學雜誌
중화핵의학잡지
CHINESE JOURNAL OF NUCLEAR MEDICINE
2011年
2期
87-91
,共5页
王浩炜%汤亚莉%石怡珍%马孝明%刘增礼
王浩煒%湯亞莉%石怡珍%馬孝明%劉增禮
왕호위%탕아리%석이진%마효명%류증례
宫颈肿瘤%钠/碘转运体%转染%肿瘤细胞,培养的%碘放射性同位素%放射性核素显像%小鼠,裸
宮頸腫瘤%鈉/碘轉運體%轉染%腫瘤細胞,培養的%碘放射性同位素%放射性覈素顯像%小鼠,裸
궁경종류%납/전전운체%전염%종류세포,배양적%전방사성동위소%방사성핵소현상%소서,라
Cervix neoplasms%Sodium/iodide symporter%Transfection%Tumor cells,cultured%Iodine radioisotopes%Radionuclide imaging%Mice,nude
目的 利用131I对转染hNIS基因的宫颈癌Hela-NIS(+)细胞移植瘤进行显像及治疗实验研究,评价hNIS基因转染介导131I治疗宫颈癌的可行性.方法 (1)利用Hela-NIS(+)细胞和未转染hNIS的Hela细胞分别建立荷宫颈癌裸鼠模型,进行131I及99TcmO4-显像,观察移植瘤的显影情况,并计算移植瘤部位与对侧相同部位的T/B比值.(2)通过腹腔注射法观察比较74,111和148 MBq131I对荷Hela-NIS(+)宫颈癌裸鼠移植瘤的抑制作用,另设不行任何治疗的对照组.用SPSS 13.0软件,样本均数间差异行t检验.结果 (1)成功构建荷Hela-NIS(+)裸鼠移植瘤与荷Hela裸鼠移植瘤模型.131I显像示荷Hela-NIS(+)裸鼠移植瘤部位明显放射性浓聚,注射后8 h T/B比值最高达17.34,而未转染hNIS基因的荷Hela裸鼠移植瘤部位始终未见明显的放射性浓聚.99TcmO4-显像示荷Hela-NIS(+)裸鼠移植瘤部位在注射后25 h内持续放射性浓聚.(2)经不同剂量的131I治疗后2~3周起,各治疗组荷Hela-NIS(+)裸鼠移植瘤生长开始受到抑制,移植瘤体积有不同程度缩小.111MBq组和148 MBq组的移植瘤抑制率差异无统计学意义(t=0.13~2.17,P>0.05),但二者均明显高于74 MBq组的移植瘤抑制率(t=2.74~5.75,P<0.05).对照组荷Hela-NIS(+)裸鼠移植瘤持续生长.结论 荷Hela-NIS(+)宫颈癌裸鼠移植瘤可明显聚集131I及99TcmO4-,且在较长时间内持续清晰显影;131I体内治疗效果显著.
目的 利用131I對轉染hNIS基因的宮頸癌Hela-NIS(+)細胞移植瘤進行顯像及治療實驗研究,評價hNIS基因轉染介導131I治療宮頸癌的可行性.方法 (1)利用Hela-NIS(+)細胞和未轉染hNIS的Hela細胞分彆建立荷宮頸癌裸鼠模型,進行131I及99TcmO4-顯像,觀察移植瘤的顯影情況,併計算移植瘤部位與對側相同部位的T/B比值.(2)通過腹腔註射法觀察比較74,111和148 MBq131I對荷Hela-NIS(+)宮頸癌裸鼠移植瘤的抑製作用,另設不行任何治療的對照組.用SPSS 13.0軟件,樣本均數間差異行t檢驗.結果 (1)成功構建荷Hela-NIS(+)裸鼠移植瘤與荷Hela裸鼠移植瘤模型.131I顯像示荷Hela-NIS(+)裸鼠移植瘤部位明顯放射性濃聚,註射後8 h T/B比值最高達17.34,而未轉染hNIS基因的荷Hela裸鼠移植瘤部位始終未見明顯的放射性濃聚.99TcmO4-顯像示荷Hela-NIS(+)裸鼠移植瘤部位在註射後25 h內持續放射性濃聚.(2)經不同劑量的131I治療後2~3週起,各治療組荷Hela-NIS(+)裸鼠移植瘤生長開始受到抑製,移植瘤體積有不同程度縮小.111MBq組和148 MBq組的移植瘤抑製率差異無統計學意義(t=0.13~2.17,P>0.05),但二者均明顯高于74 MBq組的移植瘤抑製率(t=2.74~5.75,P<0.05).對照組荷Hela-NIS(+)裸鼠移植瘤持續生長.結論 荷Hela-NIS(+)宮頸癌裸鼠移植瘤可明顯聚集131I及99TcmO4-,且在較長時間內持續清晰顯影;131I體內治療效果顯著.
목적 이용131I대전염hNIS기인적궁경암Hela-NIS(+)세포이식류진행현상급치료실험연구,평개hNIS기인전염개도131I치료궁경암적가행성.방법 (1)이용Hela-NIS(+)세포화미전염hNIS적Hela세포분별건립하궁경암라서모형,진행131I급99TcmO4-현상,관찰이식류적현영정황,병계산이식류부위여대측상동부위적T/B비치.(2)통과복강주사법관찰비교74,111화148 MBq131I대하Hela-NIS(+)궁경암라서이식류적억제작용,령설불행임하치료적대조조.용SPSS 13.0연건,양본균수간차이행t검험.결과 (1)성공구건하Hela-NIS(+)라서이식류여하Hela라서이식류모형.131I현상시하Hela-NIS(+)라서이식류부위명현방사성농취,주사후8 h T/B비치최고체17.34,이미전염hNIS기인적하Hela라서이식류부위시종미견명현적방사성농취.99TcmO4-현상시하Hela-NIS(+)라서이식류부위재주사후25 h내지속방사성농취.(2)경불동제량적131I치료후2~3주기,각치료조하Hela-NIS(+)라서이식류생장개시수도억제,이식류체적유불동정도축소.111MBq조화148 MBq조적이식류억제솔차이무통계학의의(t=0.13~2.17,P>0.05),단이자균명현고우74 MBq조적이식류억제솔(t=2.74~5.75,P<0.05).대조조하Hela-NIS(+)라서이식류지속생장.결론 하Hela-NIS(+)궁경암라서이식류가명현취집131I급99TcmO4-,차재교장시간내지속청석현영;131I체내치료효과현저.
Objective To explore the feasibility of imaging and treatment of cervical cancer xenograft model using 131I mediated by hNIS gene transfection. Methods The cervical cancer xenograft models were established with Hela-NIS( +) cells and Hela cells, respectively. Five Hela-NIS( +) xenograft models and five Hela xenograft models were dynamically imaged at 0.5, 1, 2, 4, 8, 16 and 20 h postinjection of 131I(7.4 MBq). Five Hela-NIS( +) xenograft models were imaged at 0. 5,1,2,4,8,16, 20 and 25 h postinjection of 99TcmO4-(11.1 MBq). Twenty Hela-NIS( +) cervical cancer xenograft models were randomly divided into four groups: Three 131I treating groups and one control group. The therapeutic effects of 131I at threelevels (74,111,148 MBq) were investigated following intraperitoneal injection. Results Hela-NIS( +)human cervical cancer xenografts were established successfully in nude mice. The Hela-NIS( +) xenografts significantly accumulated radioactivity after intraperitoneal injection of 131I, and the radioactivity was persistently present until 20 h postinjection, but Hela xenografts had no radioactive accumulation. The T/B value of the Hela-NIS( +) xenografts reached 17.34 at 8 h postinjection. The imaging with 99TcmO4- showed that the radioactivity was persistently present in Hela-NIS( +) xenografts for almost 25 h. The Hela-NIS( +)xenografts shrinked after 131I treatment. The inhibition ratios of tumor growth in 111 MBq and 148 MBq groups were both significantly higher than that of 74 MBq group (t: 2.74-5.75, P <0.05). Conclusions Hela-NIS( +) cervical cancer xenografts in nude mice could persistently accumulate 131I and 99TcmO4- and could be treated successfully with 131 I. 131 I treatment mediated by hNIS gene transfection could be a promising cancer treatment method.