中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2012年
2期
170-175
,共6页
肖汉方%朱国英%顾淑珠%陈晓%甘曹辉%翟江龙
肖漢方%硃國英%顧淑珠%陳曉%甘曹輝%翟江龍
초한방%주국영%고숙주%진효%감조휘%적강룡
电离辐射%血型糖蛋白A基因突变%放射诊疗工作人员%微核
電離輻射%血型糖蛋白A基因突變%放射診療工作人員%微覈
전리복사%혈형당단백A기인돌변%방사진료공작인원%미핵
Ionizing radiation%Glycophorin A mutation%Medical radiation workers%Micronuclei
目的 探讨血型糖蛋白A(GPA)基因突变频率用于辐射危险评价及预测电离辐射诱发肿瘤风险的可行性.方法 采用固定人群分层随机抽样的方法,选取上海市放射诊疗工作人员336例(按工种分为X射线影像诊断组、CT影像诊断组、介人放射学组和放射治疗组),健康对照组 112例；其中,经血型鉴定为MN杂合个体的放射诊疗工作人员为176例,健康对照者为58例.分离、固定、荧光免疫标记外周血红细胞后,应用流式细胞仪,按照BR6-1WI方法,分析GPA基因突变频率；采用胞质分裂阻断微核+3-氨基苯甲酰胺指数实验(CB微核+3AB指数实验),检测DNA损伤修复能力以反映研究对象的个体易感性.结果 放射诊疗工作人员GPA基因突变频率明显高于健康对照组(t=2.29～11.48,P＜0.05),尤其是介入放射学组的GPA NO基因突变频率明显高于X射线影像诊断组(t =2.01,P＜0.05).GPA NO基因突变频率受放射工龄、累积剂量和3AB指数的影响作用明显,而GPA NN基因突变频率仅受放射工龄影响,与累积剂量和3AB指数的相关性不明显.结论 对于职业低剂量电离辐射受照人群,GPA NO基因突变频率可较好地反映电离辐射诱发的DNA损伤效应和个体的辐射易感性,较GPA NN基因突变频率更适宜和敏感.
目的 探討血型糖蛋白A(GPA)基因突變頻率用于輻射危險評價及預測電離輻射誘髮腫瘤風險的可行性.方法 採用固定人群分層隨機抽樣的方法,選取上海市放射診療工作人員336例(按工種分為X射線影像診斷組、CT影像診斷組、介人放射學組和放射治療組),健康對照組 112例；其中,經血型鑒定為MN雜閤箇體的放射診療工作人員為176例,健康對照者為58例.分離、固定、熒光免疫標記外週血紅細胞後,應用流式細胞儀,按照BR6-1WI方法,分析GPA基因突變頻率；採用胞質分裂阻斷微覈+3-氨基苯甲酰胺指數實驗(CB微覈+3AB指數實驗),檢測DNA損傷脩複能力以反映研究對象的箇體易感性.結果 放射診療工作人員GPA基因突變頻率明顯高于健康對照組(t=2.29～11.48,P＜0.05),尤其是介入放射學組的GPA NO基因突變頻率明顯高于X射線影像診斷組(t =2.01,P＜0.05).GPA NO基因突變頻率受放射工齡、纍積劑量和3AB指數的影響作用明顯,而GPA NN基因突變頻率僅受放射工齡影響,與纍積劑量和3AB指數的相關性不明顯.結論 對于職業低劑量電離輻射受照人群,GPA NO基因突變頻率可較好地反映電離輻射誘髮的DNA損傷效應和箇體的輻射易感性,較GPA NN基因突變頻率更適宜和敏感.
목적 탐토혈형당단백A(GPA)기인돌변빈솔용우복사위험평개급예측전리복사유발종류풍험적가행성.방법 채용고정인군분층수궤추양적방법,선취상해시방사진료공작인원336례(안공충분위X사선영상진단조、CT영상진단조、개인방사학조화방사치료조),건강대조조 112례；기중,경혈형감정위MN잡합개체적방사진료공작인원위176례,건강대조자위58례.분리、고정、형광면역표기외주혈홍세포후,응용류식세포의,안조BR6-1WI방법,분석GPA기인돌변빈솔；채용포질분렬조단미핵+3-안기분갑선알지수실험(CB미핵+3AB지수실험),검측DNA손상수복능력이반영연구대상적개체역감성.결과 방사진료공작인원GPA기인돌변빈솔명현고우건강대조조(t=2.29～11.48,P＜0.05),우기시개입방사학조적GPA NO기인돌변빈솔명현고우X사선영상진단조(t =2.01,P＜0.05).GPA NO기인돌변빈솔수방사공령、루적제량화3AB지수적영향작용명현,이GPA NN기인돌변빈솔부수방사공령영향,여루적제량화3AB지수적상관성불명현.결론 대우직업저제량전리복사수조인군,GPA NO기인돌변빈솔가교호지반영전리복사유발적DNA손상효응화개체적복사역감성,교GPA NN기인돌변빈솔경괄의화민감.
Objective To explore the feasibility of using glycophorin A somatic mutation in peripheral erythrocytes,in order to evaluate the cancer risk of occupational medical exposure to ionizing radiation.Methods Totally 336 medical radiation workers were recruited as three groups (general radiation group,computer tomography group,intervention and radiation treatment group) and 112 healthy adults were selected as control by using stratified random cluster sampling method,where 176 medicalradiation workers and 58 health controls had a MN-heterozygous type.The erythrocytes were fixed and bound with fluorescent-labeled monoclonal antibody,and the glycophorin A somatic mutation frequency was assayed by a modified BR6-1W1 method using a FACScan flow cytometer.The individual susceptibility to radiation was investigated using micronuclei test and 3-Aminobenzamide index test.Results The GPA somatic mutation frequency of medical-radiation workers was significantly higher than that of healthy control ( t =2.29 - 11.48,P ＜ 0.05 ).In particular,the NO GPA aberration frequency of interventional radiology workers was much higher than that of the general medical diagnostic workers (t =2.01,P ＜ 0.05).In addition,the NO GPA variant frequency changed significantly with the years of radiation service,cumulative doses,and 3AB index.However,the NN GPA variant frequency was only associated with the years of radiation service,and no significant correlations were found between NN GPA variant frequency and cumulative dose of radiation exposure or 3AB index. Conclusions GPA mutation frequency,especially NO GPA mutation frequency could be used as a sensitive biomarker to predict the DNA damage and individual susceptibility for the population exposed to professional low-dose ionizing radiation.
