CAJ | 학술논문

目的了解手足口病(HFMD)患儿标本中分离到的柯萨奇病毒A10型(Coxsackie virus A10,CVA10)山东地方株VP1区基因特征.方法采集2008年至2009年部分HFMD病例临床标本,细胞培养方法分离病毒,提取病毒核酸,RT-PCR扩增病毒VP1区序列,生物信息学方法鉴定其基因型别.选择代表性CVA10毒株,与GenBank中检索到的其他CVA10毒株进行核苷酸和氨基酸同源性分析,并以VP1部分序列构建亲缘进化树.结果从山东省HFMD患儿的760份临床标本中分离出330株病毒,其中有17株分子定型鉴定为CVA10.CVA10山东地方株彼此间核苷酸和氨基酸的同源性分别在82.3%～100.0%和94.2%～100.0%,与CVA10原型株(Kowalik株)核苷酸和氨基酸的同源性为75.6%～76.8%和90.2%～93.2%.亲缘进化树显示,CVA10山东地方株分别属于两个不同的基因亚型.结论 CVA10也是引起HFMD的病原体之一,山东省存在CVA10两种基因亚型的共循环.
목적 료해수족구병(HFMD)환인표본중분리도적가살기병독A10형(Coxsackie virus A10,CVA10)산동지방주VP1구기인특정.방법 채집2008년지2009년부분HFMD병례림상표본,세포배양방법분리병독,제취병독핵산,RT-PCR확증병독VP1구서렬,생물신식학방법감정기기인형별.선택대표성CVA10독주,여GenBank중검색도적기타CVA10독주진행핵감산화안기산동원성분석,병이VP1부분서렬구건친연진화수.결과 종산동성HFMD환인적760빈림상표본중분리출330주병독,기중유17주분자정형감정위CVA10.CVA10산동지방주피차간핵감산화안기산적동원성분별재82.3%～100.0%화94.2%～100.0%,여CVA10원형주(Kowalik주)핵감산화안기산적동원성위75.6%～76.8%화90.2%～93.2%.친연진화수현시,CVA10산동지방주분별속우량개불동적기인아형.결론 CVA10야시인기HFMD적병원체지일,산동성존재CVA10량충기인아형적공순배.
Objective To analyze the genetic characterization of VP1 region of Coxsackie virus A10(CVA10)isolated from clinical specimens of hand, foot and mouth disease(HFMD) patients in Shandong Province. Methods Clinical specimens were collected from some of HFMD patients from 2008 to 2009. The virus was isolated by cell culture. Total RNA was extracted, and the VP1 genes of the isolates were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced. The genotypes were identified by molecular typing method and bioinformatics analysis.Homologous comparison and phylogenetic analysis of representative CVA10 strains were performed.Homologous comparison between the Shandong isolates and strains obtained from GenBank were performed and phylogenetic analysis of some representative CVA10 strains were performed. Results Three hundred and thirty viruses strains were isolated from 760 clinical specimens collected from HFMD patients, and 17 of them were identified as CVA10. The homologies of nucleotide and amino acid of the 17 CVA10 strains were 82.3%-100.0% and 94.2%-100.0%, respectively. Compared with the prototype strain of CVA10 (Kowalik/USA/2003), the homologies of nucleotide and amino acid were 75.6%-76.8% and 90.2%-93.2%, respectively. Interestingly, Shandong CVA10 strains were clustered into two distinct subgroups in the phylogenetic tree. Conclusions CVA10 is one of the causative agents of HFMD. Two independently circulating subgroups of CVA10 exist in Shandong province.