CAJ | 학술논문

目的探讨肿瘤坏死因子相关凋亡诱导配体(TRAIL)受体DR4、DR5、DcR1、DcR2在人肝细胞肝癌的原发灶及其门脉癌栓中的表达及意义.方法采用实时荧光定量逆转录-聚合酶链反应(RT-PCR)法,检测20例人肝细胞肝癌的原发灶及其门脉癌栓和20例未发生转移的原发性肝癌组织中TRAIL受体mRNA的表达水平.结果死亡受体DR4、DB5在无转移的肝癌组织分别为(3.59±0.87)、(1.98±0.54),伴有门脉癌栓的肝癌原发灶组织(分别设定为1)及其门脉癌栓组织中的表达量分别为(0.62±0.28)、(0.31±0.12),呈递减趋势(P<0.05).诱捕受体DcR1、DcR2在伴有门脉癌栓的肝癌组织中的表达量分别为(0.29±0.04)、(0.54±0.08),显著低于无转移的肝癌组织(分别设定为1)(P<0.05).而在伴发PVTT的HCC中,门脉癌栓组织与其肝癌原发灶组织中DcR的表达量差异无统计学意义(P>0.05).DR的表达水平与肿瘤的分化程度(r=0.461,P<0.05)及门静脉浸润情况(r=0.587,P<0.05)呈显著正相关.DR的表达水平与肿瘤的大小及血清甲胎蛋白(AFP)浓度无明显相关(P>0.05).结论 TRAIL死亡受体DR的表达下调可能与肝癌的恶性进展密切相关.TRAIL 途径诱导凋亡在肝癌转移过程中可能起到重要的作用.
목적 탐토종류배사인자상관조망유도배체(TRAIL)수체DR4、DR5、DcR1、DcR2재인간세포간암적원발조급기문맥암전중적표체급의의.방법 채용실시형광정량역전록-취합매련반응(RT-PCR)법,검측20례인간세포간암적원발조급기문맥암전화20례미발생전이적원발성간암조직중TRAIL수체mRNA적표체수평.결과 사망수체DR4、DB5재무전이적간암조직분별위(3.59±0.87)、(1.98±0.54),반유문맥암전적간암원발조조직(분별설정위1)급기문맥암전조직중적표체량분별위(0.62±0.28)、(0.31±0.12),정체감추세(P<0.05).유포수체DcR1、DcR2재반유문맥암전적간암조직중적표체량분별위(0.29±0.04)、(0.54±0.08),현저저우무전이적간암조직(분별설정위1)(P<0.05).이재반발PVTT적HCC중,문맥암전조직여기간암원발조조직중DcR적표체량차이무통계학의의(P>0.05).DR적표체수평여종류적분화정도(r=0.461,P<0.05)급문정맥침윤정황(r=0.587,P<0.05)정현저정상관.DR적표체수평여종류적대소급혈청갑태단백(AFP)농도무명현상관(P>0.05).결론 TRAIL사망수체DR적표체하조가능여간암적악성진전밀절상관.TRAIL 도경유도조망재간암전이과정중가능기도중요적작용.
Objective To investigate the expression and significance of TNF-related apoptosis inducing ligand (TRAIL) receptors in the primary lesions and portal vein tumor thrombi (PVTT) of the human hepatocellular carcinoma (HCC). Methods The expression of TRAIL receptors mRNA was detected by real-time fluorescence quantitative RT-PCR in the primary lesions and portal vein tuner thrombi of 20 samples of HCC, and in the tissues of 20 samples of primary hepatic carcinoma without metastasis. Results The expression of death receptors (DR4, DR5) was decreased from the primary hepatic carcinoma without metastasis [(3.59±0.87), (1.98±0.54), respectively], the primary lesions of HCC with PVTY (set it as 1 respectively) to the PVTr [(0.62±0.28), (0.31±0.12), respectively](P<0.05). The expression of decoy receptors (DcR1, DcR2) in the HCC with PVTT [(0.29±0.04), (0.54±0.06), respectively]was significantly lower than that in the primary hepatic carcinoma without metastasis (set it as 1 respectively) (P<0.05). However, the expression of decoy receptors had no significant difference between the primary lesions of HCC with PVTT (P>0.05). The expression level of decoy receptors was had significantly positive correlation with the tumor differentiation (r=0.461, P<0.05) and portal vein invasion (r= 0.587,P<0.05). However, the expression level had no significant correlation with the tumor size and serum concentration of a-fetoprotein (AFP, P>0.05 ). Conclusion Lower expression of TRAIL death re-ceptors may be closely correlated to the malignant progression of HCC. TRAIL-induced apoptosis may play an important role in the metastatic process of HCC.