中华神经医学杂志
中華神經醫學雜誌
중화신경의학잡지
CHINESE JOURNAL OF NEUROMEDICINE
2008年
11期
1179-1180
,共2页
肺炎克雷伯菌%随机扩增多态性DNA%医院感染
肺炎剋雷伯菌%隨機擴增多態性DNA%醫院感染
폐염극뢰백균%수궤확증다태성DNA%의원감염
Klebsiella pneurnoniae%Randomly amplified polymorphic DNA%Nosocomial infections
目的 采用随机扩增多态性DNA(RAPD)基因分型方法 监测肺炎克雷伯菌医院感染情况. 方法 对我院神经外科重症监护室临床分离的72株肺炎克雷伯菌进行RAPD基因分型,并通过指纹图谱比较分析,确证医院感染爆发. 结果 72株肺炎克雷伯菌共分得68型,未发生肺炎克雷伯菌医院感染局部流行. 结论 RAID基因分型方法 可对肺炎克雷伯菌进行分子流行病学调查.
目的 採用隨機擴增多態性DNA(RAPD)基因分型方法 鑑測肺炎剋雷伯菌醫院感染情況. 方法 對我院神經外科重癥鑑護室臨床分離的72株肺炎剋雷伯菌進行RAPD基因分型,併通過指紋圖譜比較分析,確證醫院感染爆髮. 結果 72株肺炎剋雷伯菌共分得68型,未髮生肺炎剋雷伯菌醫院感染跼部流行. 結論 RAID基因分型方法 可對肺炎剋雷伯菌進行分子流行病學調查.
목적 채용수궤확증다태성DNA(RAPD)기인분형방법 감측폐염극뢰백균의원감염정황. 방법 대아원신경외과중증감호실림상분리적72주폐염극뢰백균진행RAPD기인분형,병통과지문도보비교분석,학증의원감염폭발. 결과 72주폐염극뢰백균공분득68형,미발생폐염극뢰백균의원감염국부류행. 결론 RAID기인분형방법 가대폐염극뢰백균진행분자류행병학조사.
Objective To monitor nosocomial infections of Klebsiella pneumoniae by analyzing the randomly amplified polymorphic DNA (RAPD). Methods RAPD analysis was used to genotype 72 Klebsiella pneumoniae strains isolated from the intensive care unit (ICU) of the neurosurgical department, and the fingerprints were compared to confirm the outbreak of nosocomial infection of Klebsiella pneumoniae. Results 72 strains Klebsiella pneurnoniae were classified into 68 RAPD types, suggesting the absence of an outbreak of nosocomial infection of glebsiella pneurnoniae in the ICU.Conclusian RAPD genotyping can be used in the molecular epidemiological study ofKlebsiella pneurnoniae.
