CAJ | 학술논문

本文选用天然免疫活性多糖-香菇(Lentinus edodes)、银耳(Tremella fuciformis Berk)和黄芪(Astragalus membranaceus Bge)多糖,研究其对柔嫩艾美尔球虫感染鸡细胞因子IFN-γ和IL-2水平的影响.选择180只雏鸡并将其随机分为9组:3个多糖提取物添加组(LenE、TreE和AstE),3个添加提取物并免疫接种疫苗组(LenE+V、TreE+V和AstE+V),1个球虫免疫组和2个对照组(球虫感染组和非感染组).检测球虫感染后第7、14天鸡血清IFN-γ效价和脾淋巴细胞IL-2的水平.结果显示,球虫感染后第7、14天,添加多糖提取物并疫苗免疫组的血清IFN-γ效价显著高于单纯疫苗组(P＜0.01).然而,单纯提取物添加组与单纯疫苗组之间没有显著差异.3种多糖提取物相比,感染第7天后,AstE组的IFN-γ效价最高,而TreE+V组的IFN-γ的效价显著高于LenE+V组和AstE+V组.脾脏细胞IL-2产量与血清IFN-γ效价的表现基本一致.感染后第7天,提取物加疫苗免疫组的平均IL-2水平显著(P＜0.01)高于单纯疫苗组,提取物添加组的平均IL-2水平与单纯疫苗组之间没有显著差异.感染第14天后,多糖提取物添加组及多糖提取物加疫苗组的平均IL-2水平都与单纯疫苗组没有显著差异.IL-2产量在不同多糖提取物添加组间没有显著差异.本实验结果表明,多糖提取物抗球虫作用可能与刺激免疫细胞分泌IFN-γ和IL-2等细胞因子、提高T-细胞免疫应答有关;中药免疫活性多糖对球虫感染鸡有很好的免疫保护作用,当多糖与疫苗一起使用时,效果尤为明显.
본문선용천연면역활성다당-향고(Lentinus edodes)、은이(Tremella fuciformis Berk)화황기(Astragalus membranaceus Bge)다당,연구기대유눈애미이구충감염계세포인자IFN-γ화IL-2수평적영향.선택180지추계병장기수궤분위9조:3개다당제취물첨가조(LenE、TreE화AstE),3개첨가제취물병면역접충역묘조(LenE+V、TreE+V화AstE+V),1개구충면역조화2개대조조(구충감염조화비감염조).검측구충감염후제7、14천계혈청IFN-γ효개화비림파세포IL-2적수평.결과현시,구충감염후제7、14천,첨가다당제취물병역묘면역조적혈청IFN-γ효개현저고우단순역묘조(P＜0.01).연이,단순제취물첨가조여단순역묘조지간몰유현저차이.3충다당제취물상비,감염제7천후,AstE조적IFN-γ효개최고,이TreE+V조적IFN-γ적효개현저고우LenE+V조화AstE+V조.비장세포IL-2산량여혈청IFN-γ효개적표현기본일치.감염후제7천,제취물가역묘면역조적평균IL-2수평현저(P＜0.01)고우단순역묘조,제취물첨가조적평균IL-2수평여단순역묘조지간몰유현저차이.감염제14천후,다당제취물첨가조급다당제취물가역묘조적평균IL-2수평도여단순역묘조몰유현저차이.IL-2산량재불동다당제취물첨가조간몰유현저차이.본실험결과표명,다당제취물항구충작용가능여자격면역세포분비IFN-γ화IL-2등세포인자、제고T-세포면역응답유관;중약면역활성다당대구충감염계유흔호적면역보호작용,당다당여역묘일기사용시,효과우위명현.
The effects of natural immano-active polysaccharide extracts (E) from two mushrooms, Lentinus edodes (LenE) and Tremella fuciformis (TreE), and an herb, Astragalus membranaceus (AstE), on production of the cytokines IFN-γ and IL-2 were investigated in Eimeria tenella infected chickens. A total of 180 broiler chicken were assigned to nine groups: three groups fed with the extracts (LenE, TreE and AstE), three groups fed with extracts and immunized with live oecyst vaccine (LenE + V, TreE + V and AstE + V), a group immunized with oecyst vaccine, and two controls (With or without E. tenella infected). Serum IFN-γ titers and IL-2 production of splenocytes were determined at 7 and 14 days post-infection (p. i. ). The overall mean serum IFN-γ of the groups fed with the extracts and immunized with vaccine was significantly (P ＜ 0.01 ) higher compared with the vaccine group at 7 and 14 days p. i., whereas that of the groups fed with the extracts only was not significantly different from the vaccine group. Of the three extracts, AstE showed the highest IFN-γ titers at 7 days p. i., and the Tree + V group had significantly higher IFN-γ titers compared with both LenE + V and AstE + V groups. The IL-2 production of splenocytes showed a similar pattern to the serum IFN-γ titers. Seven days p. i., the overall mean IL-2 level of the groups fed with extracts and immunized with vaccine was significantly (P ＜ 0.01) higher compared with the group immunized with the oecyst vaccine only, but that of the groups fed with the extracts only were not significantly different from the vaccine group. At 14 days p. i., the overall mean IL-2 production of both the groups fed with the extracts and the groups fed with the extracts and immunized with vaccine was not significantly different from the vaccine group. A significant difference in IL-2 production of splenecytes between different extracts was not found. The use of the mushroom and herb polysaccharide extracts might enhance T-cell immune responses, characterized by IFN-γ and IL-2 secretion, against coccidiosis in chickens, and particularly when they are used in conjunction with vaccine. Thus,these immunoactive herbal polysaccharide extracts may enhance protective immunity against infections.