CAJ | 학술논문

为阐明硅提高水稻抗稻瘟病的生理机制,采用室内溶液培养试验,研究了硅对接种稻瘟病菌后水稻叶片的几丁质酶和β-1,3-葡聚糖酶活性以及总可溶性酚和木质素含量的影响.结果表明,接种稻瘟病菌能诱导几丁质酶活性的快速上升,不施硅处理的几丁质酶活性在第2 d达到第一个峰值后就开始下降,而施硅处理的几丁质酶活性则继续上升,直到第4 d才开始下降,从第4～8 d显著高于不施硅处理.β-1,3-葡聚糖酶活性在接种后的第4 d之前均上升缓慢.处理间差异不显著;第4 d后开始上升,到第8 d达到最大值;不施硅处理上升更快,显著高于施硅处理.接种稻瘟病菌能诱导水稻叶片总可溶性酚含量快速上升,施硅处理和不施硅处理分别在接种后的第3 d和第4 d达到峰值,并开始快速下降;施硅能显著提高总可溶性酚含量.水稻叶片中的木质素含量在接种后的第1d快速上升,并维持较高水平,施硅处理显著高于不施硅处理;但在感病后期(第6 d),施硅处理开始显著低于不施硅处理.
위천명규제고수도항도온병적생리궤제,채용실내용액배양시험,연구료규대접충도온병균후수도협편적궤정질매화β-1,3-포취당매활성이급총가용성분화목질소함량적영향.결과표명,접충도온병균능유도궤정질매활성적쾌속상승,불시규처리적궤정질매활성재제2 d체도제일개봉치후취개시하강,이시규처리적궤정질매활성칙계속상승,직도제4 d재개시하강,종제4～8 d현저고우불시규처리.β-1,3-포취당매활성재접충후적제4 d지전균상승완만.처리간차이불현저;제4 d후개시상승,도제8 d체도최대치;불시규처리상승경쾌,현저고우시규처리.접충도온병균능유도수도협편총가용성분함량쾌속상승,시규처리화불시규처리분별재접충후적제3 d화제4 d체도봉치,병개시쾌속하강;시규능현저제고총가용성분함량.수도협편중적목질소함량재접충후적제1d쾌속상승,병유지교고수평,시규처리현저고우불시규처리;단재감병후기(제6 d),시규처리개시현저저우불시규처리.
A series of hydroponics experiments were pedormed in controlled rice- Magnaporthe grisea pathosystem to study the effects of silicon (Si) on activities of chitinase and β - 1,3-glucanase and contents of phenolics and lignin. Regardless of silicon amendment, exochitinase and endochitinase activities had entirely consistent trend, and both rapidly increased after inoculation. Chitinase activity in Si-plants reached the first peak at 2 d after inoculation, and thereafter began to decline. However, chitinase activity in Si + plants continuously increased until 4 d after inoculation and was sig-nificantly higher than in Si-plants from 4 d to 8 d. By contrast, silicon application decreased β - 1, 3-glucanase activity in rice leaves infected by M. grisea. Contents of total soluble phenolics and lignin in Si + plants were significantly higher than in Si-plants. However, lignin content in Si + plants was significantly lower than in Si-plants at 6 d after inoculation. These findings support the hypothesis that silicon is closely involved in induced resistance to rice blast.