贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2009年
12期
126-129
,共4页
周文美%王照波%徐子婷%胡晓瑜
週文美%王照波%徐子婷%鬍曉瑜
주문미%왕조파%서자정%호효유
玉米芯%毛壳菌%木聚糖%木聚糖酶%低聚木糖
玉米芯%毛殼菌%木聚糖%木聚糖酶%低聚木糖
옥미심%모각균%목취당%목취당매%저취목당
corncob%Chaetomium globosum%xylan%xylanase%xylooligosaccharide
利用玉米芯生产粗木聚糖,再用球毛壳菌 ACCC30566木聚糖酶液酶解粗木聚糖,获得低聚木糖,最后对低聚木糖进行提取纯化,并用薄层层析法初步测定了低聚木糖成分.结果表明:球毛壳菌ACCC30566木聚糖酶酶解产物主要成分为木二糖、木三糖、木四糖等低聚木糖,葡萄糖含量较少.该菌株所产木聚糖酶成分较纯,纤维素酶含量较低.可以用来生产低聚木糖.
利用玉米芯生產粗木聚糖,再用毬毛殼菌 ACCC30566木聚糖酶液酶解粗木聚糖,穫得低聚木糖,最後對低聚木糖進行提取純化,併用薄層層析法初步測定瞭低聚木糖成分.結果錶明:毬毛殼菌ACCC30566木聚糖酶酶解產物主要成分為木二糖、木三糖、木四糖等低聚木糖,葡萄糖含量較少.該菌株所產木聚糖酶成分較純,纖維素酶含量較低.可以用來生產低聚木糖.
이용옥미심생산조목취당,재용구모각균 ACCC30566목취당매액매해조목취당,획득저취목당,최후대저취목당진행제취순화,병용박층층석법초보측정료저취목당성분.결과표명:구모각균ACCC30566목취당매매해산물주요성분위목이당、목삼당、목사당등저취목당,포도당함량교소.해균주소산목취당매성분교순,섬유소매함량교저.가이용래생산저취목당.
Xylooligosaccharide was hydrolyzed from crude xylan produced from corncobs by xylanase of ACCC30566 strain of Chaetomium globosum and the xylooligosaccharide composition was determined by thin layer chromatography. The results showed that the main composition of enzymatic products by xylanase of ACCC30566 strain of Chaetomium globosum were xylobiose, xylotriose, xylotetraose and a little glucose. The composition of xylanase produced by ACCC30566 stain is purity and its cellulose content is lower. Therefore, the ACCC30566 strain of Chaetomium globosum can be used in production of Xylooligosaccharide.