CAJ | 학술논문

[目的]纹状体Parv中间神经元被认为是连接纹状体传入和传出神经元之间的枢纽.本实验旨在观察证实纹状体Panr神经元胞体、树突和轴突的形态学特征和在纹状体的分布及其与纹状体不同区域之间的关系.[方法]对成年雄性SD大鼠常规灌注固定和取脑;半导体冰冻切片之后进行免疫组化PAP方法单标记和双标记;光镜观察阳性神经元的形态并进行计数和计算其百分比;应用SPSS软件统计处理.[结果]Parv阳性神经元在纹状体呈现散在不均匀分布,在纹状体背外侧区的分布多于其它区域(P<0.001);Parr阳性神经元在Matrix间区分布的百分比显著高于Patch间区(82.0%比18.0%,P<0.001);纹状体Parv阳性细胞为典型的中等大小的中间神经元(平均直径11.68 μm),胞体呈多边形或卵圆形,外侧区胞体直径大于内侧区(P<0.01);阳性神经元树突密集,表面光滑和未见树突棘;神经元轴突细长,在纹状体可见其分支.[结论] Parv阳性中间神经元在纹状体背外侧区的密集分布、高百分比聚集于Matrix间区和典型的中间神经元特征,提示其可能对纹状体不同区域的投射神经元具有选择性和特异性作用.
[목적]문상체Parv중간신경원피인위시련접문상체전입화전출신경원지간적추뉴.본실험지재관찰증실문상체Panr신경원포체、수돌화축돌적형태학특정화재문상체적분포급기여문상체불동구역지간적관계.[방법]대성년웅성SD대서상규관주고정화취뇌;반도체빙동절편지후진행면역조화PAP방법단표기화쌍표기;광경관찰양성신경원적형태병진행계수화계산기백분비;응용SPSS연건통계처리.[결과]Parv양성신경원재문상체정현산재불균균분포,재문상체배외측구적분포다우기타구역(P<0.001);Parr양성신경원재Matrix간구분포적백분비현저고우Patch간구(82.0%비18.0%,P<0.001);문상체Parv양성세포위전형적중등대소적중간신경원(평균직경11.68 μm),포체정다변형혹란원형,외측구포체직경대우내측구(P<0.01);양성신경원수돌밀집,표면광활화미견수돌극;신경원축돌세장,재문상체가견기분지.[결론] Parv양성중간신경원재문상체배외측구적밀집분포、고백분비취집우Matrix간구화전형적중간신경원특정,제시기가능대문상체불동구역적투사신경원구유선택성화특이성작용.
[Objective ] Intrastriatal Parr interneurons are considered to be the junction between striatal input and output neurons. This study purposes to confirm the morphology of Parr neurons and their distribution in striatum. [Method] Adult male SD rats were perfused and the brains were removed. The sections were conducted with a semiconductor-frozen microtome, then single labeling and double labeling were immunocytochemically conducted with PAP technique. Positive neurons were observed, counted, and calculated, and analyzed with SPSS. [Results] The distribution of Parr positive neurons in striatum was inhomogeueous, and was the most dense in the dorsal lateral area (P<0.001) ; the distribution of Parr positive neurons in matrix compartment was apparently more than patch compartment (82.0% vs 18.0%, P<0.001); Parr positive neurons in striaturn were medium-sized cell bodies with polygonal or oval in shape (mean diameter is 11.68 μm), and were larger in the lateral area than that in the medial area (P<0.01) ; the positive dendrites were dense and smooth without dendrite spines; the positive axons 1 were slender and their collaterals extended in striatum. [Conclusion] The characteristic of Parr positive neurons collecting mostly in lateral striatum and matrix eompartment, as well as their traits of intemeurons, indicated that they would affect the striatal projection neuronal function.