基因组蛋白质组与生物信息学报（英文版）
基因組蛋白質組與生物信息學報（英文版）
기인조단백질조여생물신식학보（영문판）
GENOMICS、PROTEOMICS & BIOINFORMATICS
2011年
6期
211-217
,共7页
Stevia%miRNA%computational prediction%expression analysis%target prediction%stem-loop reverse transcription
MicroRNAs (miRNAs) usually contain 19-24 nucleotides and have been identified as important eukaryotic gene regulators.Applications of various computational approaches have simplified the task by predicting miRNAs from available sequence data sources.In this study,we identified a conserved miR414 from a computational analysis of EST sequence data available from Stevia rebaudiana.In addition,we also identified six conserved miRNAs namely miR169,miR319,miR414,miR164,miR167 and miR398 using stem-loop RT-PCR analysis.Hence,miR414 was commonly identified using both methods.The expression analysis of these miRNAs documented their roles in growth and development of Stevia.Furthermore,the detected miRNAs were found to target genes involved in plant growth,development,metabolism and signal transduction.This is the first study reporting these conserved miRNAs and their expression in Stevia.
