CAJ | 학술논문

血吸虫病是一种危害严重的重要人兽共患寄生虫病.由于多年积极有效的防治,血吸虫的感染率和感染度明显下降,传统的病原学和免疫学诊断方法已无法满足疫区查病的需要.近年来,国内外研究者们发展了一些针对血吸虫来源DNA的核酸诊断技术,显示了很好的应用前景.该文就核酸检测在血吸虫病诊断中的最新进展进行综述,主要包括靶DNA片段的选择、检测方法及效果评价、存在的问题等方面.
혈흡충병시일충위해엄중적중요인수공환기생충병.유우다년적겁유효적방치,혈흡충적감염솔화감염도명현하강,전통적병원학화면역학진단방법이무법만족역구사병적수요.근년래,국내외연구자문발전료일사침대혈흡충래원DNA적핵산진단기술,현시료흔호적응용전경.해문취핵산검측재혈흡충병진단중적최신진전진행종술,주요포괄파DNA편단적선택、검측방법급효과평개、존재적문제등방면.
Schistosomiasis is a serious parasitic disease.The infection rate and degree ot infection were decreased significantly due to the prevention and treatment of schistosomiasis for years,but the diagnostic methods,such as traditional parasitological method and immunological detection,have been unable to meet the needs in endemic areas so far.During recent years,a series of diagnostic methods for detection of the nucleic acid derived from parasitic schistosome has been developed with a wide perspective for application.The latest progress on nucleic acid diagnostics of schistosomiasis was reviewed in the present paper,including the choice of target DNA fragments,detection formats,problem and prospect.