中国基层医药
中國基層醫藥
중국기층의약
CHINESE JOURNAL OF PRIMARY MEDICINE AND PHARMACY
2010年
1期
4-6,插5
,共4页
梁艳冰%唐皓%陈志斌%李振宇%徐嘉%袁运生%马中富
樑豔冰%唐皓%陳誌斌%李振宇%徐嘉%袁運生%馬中富
량염빙%당호%진지빈%리진우%서가%원운생%마중부
全身炎症反应综合征%细胞因子%信号转录抑制因子
全身炎癥反應綜閤徵%細胞因子%信號轉錄抑製因子
전신염증반응종합정%세포인자%신호전록억제인자
Systemic inflammatory response syndrome%Cytokine%suppressor of cytokines signaling protein
目的 探讨全身炎症反应综合征(SIRS)患者外周血单个核细胞(PBMCs)细胞因子信号转导抑制因子-1/3(SOCS-1、SOCS-3)表达的意义.方法 选择20例SIRS患者(SIRS组)、16例健康人(对照组),采用密度梯度离心法从外周血分离出PBMCs,以逆转录-聚合酶链法、Western-Blotting法检测PBMCs SOCS-1与SOCS-3基因及蛋白表达.结果 SIRS组和对照组PBMCs SOCS-1 mRNA表达量分别为(0.642±0.112)、(0.458±0.044)(P<0.05),SOCS-1蛋白表达量为(0.471±0.041)、(0.488±0.019)(P>0.05),SOCS-3mRNA表达量为(0.989±0.324)、(0.459±0.046)(P<0.05),SOCS-3蛋白表达量为(0.426±0.015)、(0.400±0.025)(P>0.05).结论 SIRS患者PBMCs SOCS-1与SOCS-3 mRNA表达上调,可能参与了SIRS的发生发展.
目的 探討全身炎癥反應綜閤徵(SIRS)患者外週血單箇覈細胞(PBMCs)細胞因子信號轉導抑製因子-1/3(SOCS-1、SOCS-3)錶達的意義.方法 選擇20例SIRS患者(SIRS組)、16例健康人(對照組),採用密度梯度離心法從外週血分離齣PBMCs,以逆轉錄-聚閤酶鏈法、Western-Blotting法檢測PBMCs SOCS-1與SOCS-3基因及蛋白錶達.結果 SIRS組和對照組PBMCs SOCS-1 mRNA錶達量分彆為(0.642±0.112)、(0.458±0.044)(P<0.05),SOCS-1蛋白錶達量為(0.471±0.041)、(0.488±0.019)(P>0.05),SOCS-3mRNA錶達量為(0.989±0.324)、(0.459±0.046)(P<0.05),SOCS-3蛋白錶達量為(0.426±0.015)、(0.400±0.025)(P>0.05).結論 SIRS患者PBMCs SOCS-1與SOCS-3 mRNA錶達上調,可能參與瞭SIRS的髮生髮展.
목적 탐토전신염증반응종합정(SIRS)환자외주혈단개핵세포(PBMCs)세포인자신호전도억제인자-1/3(SOCS-1、SOCS-3)표체적의의.방법 선택20례SIRS환자(SIRS조)、16례건강인(대조조),채용밀도제도리심법종외주혈분리출PBMCs,이역전록-취합매련법、Western-Blotting법검측PBMCs SOCS-1여SOCS-3기인급단백표체.결과 SIRS조화대조조PBMCs SOCS-1 mRNA표체량분별위(0.642±0.112)、(0.458±0.044)(P<0.05),SOCS-1단백표체량위(0.471±0.041)、(0.488±0.019)(P>0.05),SOCS-3mRNA표체량위(0.989±0.324)、(0.459±0.046)(P<0.05),SOCS-3단백표체량위(0.426±0.015)、(0.400±0.025)(P>0.05).결론 SIRS환자PBMCs SOCS-1여SOCS-3 mRNA표체상조,가능삼여료SIRS적발생발전.
Objective To observe the mRNA and protein expression of SOCS-1 and SOCS-3 in the peripheral blood mononuclear cells(PBMCs) in patients with systemic inflammatory response syndrome(SIRS) and explore its clinical significance.Methods Peripheral blood of 20 patients with SIRS and 16 healthy people wag collected and the PBMCs were isolated by centrifugafion and Ficoll-Hypaque sedimentation.The mRNA of SOCS-1 and SOCS-3 was determined by reverse transcription-polymerase chain reaction(RT-PCR).And the protein content of SOCS-1 and SOCS-3 was determined by Western Blotting.Results The SOCS-1 mRNA expression in the PBMCs of SIRS group (0.642±0.112) was significant higher than that of control group(0.458±0.044)(P<0.05).There was no significant difference between the PBMCs,SOCS-1 content of control group(0.488±0.019) and SIRS group (0.471±0.041).The SOCS-3 mRNA expression in the PBMCs of SIRS group(0.989±0.324) was higher than that control group(0.459±0.046)(P<0.05).There was no significant difference between the PBMCs'SOCS-3 content of control group(0.400±0.025) and SIRS group(0.426±0.015).Conclusions The mRNA expression of SOCS-1 and SOCS-3 in the PBMCs were higher in SIRS patients.The SOCS-1 and SOCS-3 may participate in the development of SIRS.
