CAJ | 학술논문

目的探讨膀胱移行细胞癌组织环氧合酶-2(COX-2)和基质金属蛋白酶-2(MMP-2)的表达及其相关性.方法应用Western blot方法检测42例膀胱移行细胞癌组织(其中Ta～T1期18例,T2～T4期24例;G1级12例,G2级19例,G3级11例;有转移26例,无转移16例)和8例正常对照膀胱组织COX-2及MMP-2蛋白的表达,并分析其与肿瘤分级、分期和转移的关系以及两者的相关性.结果 COX-2在Ta～T1期相对表达量为0.729±0.458,T2～T4期为1.248±0.425,均显著高于正常对照膀胱组织(0.310±0.149)(t值分别为3.56、4.13,均P＜0.05);COX-2在G1、G2、G3级相对表达量分别为0.616±0.486、1.055±0.406、1.422±0.341,均显著高于正常对照膀胱组织(0.310±0.149)(F=5.98,均P＜0.05).MMP-2在Ta～T1期相对表达量为0.844±0.345,T2～T4期为1.458±0.463,均显著高于正常对照膀胱组织(0.460±0.213)(t值分别为3.91、4.83,均P＜0.05);MMP-2在G1、G2、G3级相对表达量分别为0.736±0.355、1.197±0.394、1.692±0.373,均显著高于正常对照膀胱组织(0.460±0.213)(F=6.35,均P＜0.05).在有转移及无转移肿瘤组织中COX-2表达分别为1.246±0.426和0.668±0.421,MMP-2为1.430±0.461和0.814±0.341(t值分别为5.89、6.27,均P＜0.01).COX-2与MMP-2的表达呈显著正相关(r=0.648,P＜0.01).结论 COX-2与MMP-2在膀胱移行细胞癌组织高表达,且随肿瘤恶性程度增高而表达增加,二者与膀胱移行细胞癌的发生、发展具有相关性.
목적 탐토방광이행세포암조직배양합매-2(COX-2)화기질금속단백매-2(MMP-2)적표체급기상관성.방법 응용Western blot방법검측42례방광이행세포암조직(기중Ta～T1기18례,T2～T4기24례;G1급12례,G2급19례,G3급11례;유전이26례,무전이16례)화8례정상대조방광조직COX-2급MMP-2단백적표체,병분석기여종류분급、분기화전이적관계이급량자적상관성.결과 COX-2재Ta～T1기상대표체량위0.729±0.458,T2～T4기위1.248±0.425,균현저고우정상대조방광조직(0.310±0.149)(t치분별위3.56、4.13,균P＜0.05);COX-2재G1、G2、G3급상대표체량분별위0.616±0.486、1.055±0.406、1.422±0.341,균현저고우정상대조방광조직(0.310±0.149)(F=5.98,균P＜0.05).MMP-2재Ta～T1기상대표체량위0.844±0.345,T2～T4기위1.458±0.463,균현저고우정상대조방광조직(0.460±0.213)(t치분별위3.91、4.83,균P＜0.05);MMP-2재G1、G2、G3급상대표체량분별위0.736±0.355、1.197±0.394、1.692±0.373,균현저고우정상대조방광조직(0.460±0.213)(F=6.35,균P＜0.05).재유전이급무전이종류조직중COX-2표체분별위1.246±0.426화0.668±0.421,MMP-2위1.430±0.461화0.814±0.341(t치분별위5.89、6.27,균P＜0.01).COX-2여MMP-2적표체정현저정상관(r=0.648,P＜0.01).결론 COX-2여MMP-2재방광이행세포암조직고표체,차수종류악성정도증고이표체증가,이자여방광이행세포암적발생、발전구유상관성.
Objective To evaluate the level of MMP-2 and COX-2 Protein in bladder transitional cell carcinoma tissue and explore their relationships. Methods A total of 42 patients with bladder transitional cell carcinoma, including Ta-T1 (n=18), T2-T4 (n=24), G1(n=12), G2 (n=19), G3 (n=11), metastasis (n=26) and without metastasis (n=16), were enrolled in the study. Eight normal bladder tissues were selected as control group. Western blotting was performed todetect the mRNA level of MMP-2 and COX-2. Results The relative COX-2 protein level of Ta-T1 (0.729±0.458), T2-T4 (1.248±0.425), G1 (0.61±0.486), G2 (1.055±0.406), G3 (1.422±0.341) were all higher than that of the control group significantly (0.31±0.149, t = 3.56, 4.13; F = 5.98, P ＜0.05). The relative MMP-2 protein level of Ta-T1 (0.844±0.345), T2-T4 (1.458±0.463), G1 (0.971 ±0.370), G2(1.445±0.378), G3 (1.755±0.387) were all higher than that of the control group (0.460±0.213, t = 3.91, 4.83;F = 6.35, P ＜0.05). The COX-2 and MMP-2 protein level in tumor tissues with and without metastasis were 1.246±0.426 vs 0.668±0.421, 1.430±0.461 vs 0.814±0.341, t = 5.89, 6.27, P ＜0.01, respectively. The level of COX-2 protein was positively correlated with MMP-2 positively (r =0.48, P ＜0.01). Conclusion MMP-2 and COX-2 protein are highly expressed in bladder transitional cell carcinoma tissue and their expression is positively correlated with the malignant degree. MMP-2 and COX-2 might play a synergetic role in the carcinogenesis of bladder transitional cell carcinoma.