中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2008年
5期
334-336
,共3页
邹小明%茹江江%高勇%王武军
鄒小明%茹江江%高勇%王武軍
추소명%여강강%고용%왕무군
体外循环%再灌注损伤%脑%基因表达
體外循環%再灌註損傷%腦%基因錶達
체외순배%재관주손상%뇌%기인표체
Extracorporeal circulation%Reperfusion injury%Brain%Gene expression
目的 探讨脑红蛋白(Ngb)mRNA在深低温停循环(DHCA)下的表达及意义.方法 建立DHCA犬模型,分别于体外循环即刻、停循环即刻、停循环60min、复温再灌注45 min,取实验犬脑皮质迅速冻存,待实验结束后统一行实时荧光PCR定量检测Ngb mRNA的表达.结果 体外循环后,特别是降温后Ngb,mRNA的拷贝均升高,至停循环60min达最高值(5.7±2.2)×108拷贝数/μl,复温再灌注后轻微下降.不同时间点Ngb mRNA的拷贝浓度间差异有统计学意义(F=8.565,P=0.1703).停循环60min时以及复温再灌注45 min后Ngb mRNA的拷贝数均显著高于体外循环即刻时(P<0.048).结论 DHCA中缺氧可诱导Ngb mRNA的表达,随着DHCA时间的延长,Ngb mRNA表达升高,复灌后表达下降.说明缺氧是决定Ngb mRNA表达高低的主要因素.
目的 探討腦紅蛋白(Ngb)mRNA在深低溫停循環(DHCA)下的錶達及意義.方法 建立DHCA犬模型,分彆于體外循環即刻、停循環即刻、停循環60min、複溫再灌註45 min,取實驗犬腦皮質迅速凍存,待實驗結束後統一行實時熒光PCR定量檢測Ngb mRNA的錶達.結果 體外循環後,特彆是降溫後Ngb,mRNA的拷貝均升高,至停循環60min達最高值(5.7±2.2)×108拷貝數/μl,複溫再灌註後輕微下降.不同時間點Ngb mRNA的拷貝濃度間差異有統計學意義(F=8.565,P=0.1703).停循環60min時以及複溫再灌註45 min後Ngb mRNA的拷貝數均顯著高于體外循環即刻時(P<0.048).結論 DHCA中缺氧可誘導Ngb mRNA的錶達,隨著DHCA時間的延長,Ngb mRNA錶達升高,複灌後錶達下降.說明缺氧是決定Ngb mRNA錶達高低的主要因素.
목적 탐토뇌홍단백(Ngb)mRNA재심저온정순배(DHCA)하적표체급의의.방법 건립DHCA견모형,분별우체외순배즉각、정순배즉각、정순배60min、복온재관주45 min,취실험견뇌피질신속동존,대실험결속후통일행실시형광PCR정량검측Ngb mRNA적표체.결과 체외순배후,특별시강온후Ngb,mRNA적고패균승고,지정순배60min체최고치(5.7±2.2)×108고패수/μl,복온재관주후경미하강.불동시간점Ngb mRNA적고패농도간차이유통계학의의(F=8.565,P=0.1703).정순배60min시이급복온재관주45 min후Ngb mRNA적고패수균현저고우체외순배즉각시(P<0.048).결론 DHCA중결양가유도Ngb mRNA적표체,수착DHCA시간적연장,Ngb mRNA표체승고,복관후표체하강.설명결양시결정Ngb mRNA표체고저적주요인소.
Objective To investigate the value of the neuroglobin(Ngb) related to brain injury was explored in the diagnosis, therapy and prognostic assessment of brain injury due to DHCA. Methods DHCA model was established in ten healthy adult dogs. The cerebral cortex samples were obtained and frozen for real-time fluorescence quantitative PCR (RT-PCR) for Ngb mRNA ment after extractorporeal circulation establishment, 0 and 60 minutes after circulatory arrest, and 45 minutes of reperfusion. Results The establishment of extracorporeal circulation resulted in increased Ngb mRNA expression, which was especially obvious after temperature reduction, and at 60 min after DHCA its expression reached the peak level, [±S= (5.7±2.2) ×108 copy number/μg] followed by slight decrease after body temperature recovery. The concentraion of Ngb mRNA showed significant differences at different time points( F =8.565, P = 0.003). Ngb mRNA level at 60 minutes of DHCA and at 45 minutes of reperfusion was significantly higher than that measured before extracorporeal circulatio establishment and at the start of DHCA(P<0.048). Conclusion Anoxia in DHCA cam induce the Ngb mRNA expression. As DHCA was prolonged, Ngb mRNA level kept increasing but the increment was of gradually lowered magnitudes.
