中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
9期
588-592
,共5页
汪习成%樊移山%周曾全%李惠琴%张政%张彤%王丽%杨欣平%毛霖%谢荣慧%雷素云%吴昊%王福生
汪習成%樊移山%週曾全%李惠琴%張政%張彤%王麗%楊訢平%毛霖%謝榮慧%雷素雲%吳昊%王福生
왕습성%번이산%주증전%리혜금%장정%장동%왕려%양흔평%모림%사영혜%뢰소운%오호%왕복생
HIV-1%获得性免疫缺陷综合征%树突细胞
HIV-1%穫得性免疫缺陷綜閤徵%樹突細胞
HIV-1%획득성면역결함종합정%수돌세포
HIV-1%Acquired immunodeficiency syndrome%Dendritic cells
目的 观察病情不同的HIV/AIDS患者外周血髓样树突状细胞(mDC)上B7-H1表达频率和疾病进展的关系.方法 采集2006年3月至2008年4月北京佑安医院门诊或住院的82例未经抗逆转录病毒治疗(ART)的HIV/AIDS患者、28例ART病毒学完全应答者(CR)和14名健康人的外周血,采用流式细胞术分析mDC上B7-H1表达频率.所有病例均用流式细胞仪检测外周血CD4细胞计数,用bDNA法检测血浆HIV-1病毒载量.结果 AIDS患者外周血mDC上B7-H1表达频率为14.15%±2.63%,HIV无症状感染者为3.31%±0.51%,健康对照为0.52%±0.10%,显示AIDS患者>HIV感染者>健康对照(均P<0.05).长期不进展者(LTNP)和典型进展者(TP)mDC上B7-H1的表达频率分别是3.12%±1.14%和8.12%±1.37%,均显著高于健康对照组(均P<0.05),且TP患者高于LTNP(P=0.001);HIV/AIDS患者mDC上B7-H1表达频率与CD4细胞计数呈显著负相关,而与血浆病毒载量显著正相关(r值分别为-0.631及0.482,均P<0.01).28例CR患者mDC上B7-H1表达频率为6.59%±1.43%,显著低于AIDS患者(P<0.01).CR患者中,CD4细胞计数<250个/μl的患者mDC上B7-H1表达频率高于CD4细胞计数>250个/μl的患者(P<0.001).ART纵向观察结果显示,6例患者在14-52周的抗病毒治疗后,血浆病毒载量均显著降低,有不同程度的CD4细胞计数升高,而mDC上B7-H1表达有不同程度降低,CD4细胞计数显著升高的病人mDC上B7-H1表达显著降低.结论 共刺激分子B7-H1在HIV/AIDS患者外周血mDC上高表达,并与疾病进展密切相关,是疾病进展的指标.
目的 觀察病情不同的HIV/AIDS患者外週血髓樣樹突狀細胞(mDC)上B7-H1錶達頻率和疾病進展的關繫.方法 採集2006年3月至2008年4月北京祐安醫院門診或住院的82例未經抗逆轉錄病毒治療(ART)的HIV/AIDS患者、28例ART病毒學完全應答者(CR)和14名健康人的外週血,採用流式細胞術分析mDC上B7-H1錶達頻率.所有病例均用流式細胞儀檢測外週血CD4細胞計數,用bDNA法檢測血漿HIV-1病毒載量.結果 AIDS患者外週血mDC上B7-H1錶達頻率為14.15%±2.63%,HIV無癥狀感染者為3.31%±0.51%,健康對照為0.52%±0.10%,顯示AIDS患者>HIV感染者>健康對照(均P<0.05).長期不進展者(LTNP)和典型進展者(TP)mDC上B7-H1的錶達頻率分彆是3.12%±1.14%和8.12%±1.37%,均顯著高于健康對照組(均P<0.05),且TP患者高于LTNP(P=0.001);HIV/AIDS患者mDC上B7-H1錶達頻率與CD4細胞計數呈顯著負相關,而與血漿病毒載量顯著正相關(r值分彆為-0.631及0.482,均P<0.01).28例CR患者mDC上B7-H1錶達頻率為6.59%±1.43%,顯著低于AIDS患者(P<0.01).CR患者中,CD4細胞計數<250箇/μl的患者mDC上B7-H1錶達頻率高于CD4細胞計數>250箇/μl的患者(P<0.001).ART縱嚮觀察結果顯示,6例患者在14-52週的抗病毒治療後,血漿病毒載量均顯著降低,有不同程度的CD4細胞計數升高,而mDC上B7-H1錶達有不同程度降低,CD4細胞計數顯著升高的病人mDC上B7-H1錶達顯著降低.結論 共刺激分子B7-H1在HIV/AIDS患者外週血mDC上高錶達,併與疾病進展密切相關,是疾病進展的指標.
목적 관찰병정불동적HIV/AIDS환자외주혈수양수돌상세포(mDC)상B7-H1표체빈솔화질병진전적관계.방법 채집2006년3월지2008년4월북경우안의원문진혹주원적82례미경항역전록병독치료(ART)적HIV/AIDS환자、28례ART병독학완전응답자(CR)화14명건강인적외주혈,채용류식세포술분석mDC상B7-H1표체빈솔.소유병례균용류식세포의검측외주혈CD4세포계수,용bDNA법검측혈장HIV-1병독재량.결과 AIDS환자외주혈mDC상B7-H1표체빈솔위14.15%±2.63%,HIV무증상감염자위3.31%±0.51%,건강대조위0.52%±0.10%,현시AIDS환자>HIV감염자>건강대조(균P<0.05).장기불진전자(LTNP)화전형진전자(TP)mDC상B7-H1적표체빈솔분별시3.12%±1.14%화8.12%±1.37%,균현저고우건강대조조(균P<0.05),차TP환자고우LTNP(P=0.001);HIV/AIDS환자mDC상B7-H1표체빈솔여CD4세포계수정현저부상관,이여혈장병독재량현저정상관(r치분별위-0.631급0.482,균P<0.01).28례CR환자mDC상B7-H1표체빈솔위6.59%±1.43%,현저저우AIDS환자(P<0.01).CR환자중,CD4세포계수<250개/μl적환자mDC상B7-H1표체빈솔고우CD4세포계수>250개/μl적환자(P<0.001).ART종향관찰결과현시,6례환자재14-52주적항병독치료후,혈장병독재량균현저강저,유불동정도적CD4세포계수승고,이mDC상B7-H1표체유불동정도강저,CD4세포계수현저승고적병인mDC상B7-H1표체현저강저.결론 공자격분자B7-H1재HIV/AIDS환자외주혈mDC상고표체,병여질병진전밀절상관,시질병진전적지표.
Objective To investigate the features of B7-H1 expression on peripheral myeloid dendritic cells (mDCs) in patients with HIV infection and evaluate the correlations between B7-H1expression and disease progression. Methods Peripheral blood samples from 82 treatment-naive patients with HIV infection, 28 viral complete responders (CRs) under antiretroviral therapy (ART) and 14 healthy controls (HCs) were collected. Flow cytometry was applied to investigate the expression of B7-H1 on mDCs and CD4 cell counts. Plasma HIV-1 viral load was detected by bDNA. Results The frequency of B7-H1expression on mDCs were 14. 15%±2.63%, 3. 31% ±0.51% and 0.52%±0. 10% in AIDS patients,asymptomatic HIV infected individuals and HCs respectively. As compared with HCs, B7-H1 was significantly up-regulated on mDCs in HIV/AIDS patients. The order was as follows: AIDS patients >asymptomatic HIV infected individuals > HCs ( all P < 0. 05 ). Interestingly, the expression of B7-H1 on mDCs in long-term nonprogressors (LTNPs) was 3. 12% ±1.14%. And it was lower than that in typical progressors (TPs) [8. 12% ±1.37% (P=0.001)]. Moreover, the expression of B7-H1 was negatively correlated with CD4 cell counts and positively correlated with plasma viral load in these patients (r =-0.631, P <0.01 and r =0.482, P <0.01 respectively). The expression of B7-H1 on mDCs was significantly lower in ART complete responders than that in AIDS patients (6. 59% ± 1.43% vs 14. 15% ±2. 63% ) ( P <0. 01 ). Expression of B7-H1 on mDCs decreased markedly in patients whose CD4 cell counts greatly elevated after a successful antiretroviral treatment. Conclusion The expression of B7-H1 on mDCs is significantly up-regalated in HIV/AIDS patients. With a close correlation with disease status, it acts as a marker of disease progression.
