CAJ | 학술논문

肌酐酰氨基水解酶是酶法分析血清肌酐浓度的关键酶。本实验室从空气中分离到能分解肌酐的菌株K9510、K9511和K9512，其中K9510菌株初步分类鉴定为假单胞菌(Pseudomonas sp.)。菌株产酶条件优化研究结果表明：菌株在底物或底物类似物的诱导下产酶；混合金属离子溶液对菌株产酶有促进作用。菌株产肌酐酰氨基水解酶最适培养基组成为：肌酐9g、酵母提取物1.5g、麦芽汁0.9g、NH4Cl 0.5g、定容1L。适量混合金属离子溶液，用0.1mol/LpH5.5磷酸缓冲液配制。在250mL三角瓶中装50mL培养基,在250r/min的旋转摇床上35℃振荡培养33h，在此条件下菌株产酶量可达1.0u/mL发酵液。
기항선안기수해매시매법분석혈청기항농도적관건매。본실험실종공기중분리도능분해기항적균주K9510、K9511화K9512，기중K9510균주초보분류감정위가단포균(Pseudomonas sp.)。균주산매조건우화연구결과표명：균주재저물혹저물유사물적유도하산매；혼합금속리자용액대균주산매유촉진작용。균주산기항선안기수해매최괄배양기조성위：기항9g、효모제취물1.5g、맥아즙0.9g、NH4Cl 0.5g、정용1L。괄량혼합금속리자용액，용0.1mol/LpH5.5린산완충액배제。재250mL삼각병중장50mL배양기,재250r/min적선전요상상35℃진탕배양33h，재차조건하균주산매량가체1.0u/mL발효액。
From air bacteria capable of decomposing creatinine, three single independent strains K9510、K9511 and K9512 have been isolated. The highest creatinine amidohydrolase (EC 3.5.2.10; creatininase) producing strain K9510 was screened out. The strain K9510 was identified as Pseudomonas sp. The results of culture condition for creatininase formation by strain K9510 were obtained as follows: creatinine and creatine were found to be the effective inducers for enzyme formation; the solution of mixed metallic salts could stimulate cell growth and enzyme formation. The suitable medium for creatininase formation was consisted of 0.9% creatinine、 0. 15% yeast extract、 0. 09% malt extract、0.05% NH4C1 and some amount of the solution of mixed metallic salts at pH5. 5. When the bacterium was grown in 250mL conic flask containing 50mL of the medium mentioned above on the rotary shaker(250r/min) at 35℃ for 33 h, about 50 u creatininase was obtained.