CAJ | 학술논문

为了研究H5N1 DNA疫苗对小鼠和鸡的保护效率,用H5N1禽流感病毒HA DNA疫苗免疫BALB/c小鼠和SPF鸡.小鼠和鸡分别经电穿孔和肌肉注射免疫两次,间隔为3周.二次免疫后,用致死量的同源病毒进行攻毒实验.空白对照组在攻毒后全部死亡,而经电穿孔免疫的小鼠和鸡均获得了完全的保护,并能有效地抑制病毒在小鼠肺脏和鸡泄殖腔的繁殖.同时,电穿孔免疫的小鼠和鸡均产生了高水平的特异性抗体.经电穿孔免疫的小鼠攻毒后CTL反应明显加强.这些结果表明,HADNA疫苗能有效地保护小鼠和鸡对禽流感病毒的感染,同时也表明电穿孔免疫是DNA疫苗免疫的有效途径之一.
위료연구H5N1 DNA역묘대소서화계적보호효솔,용H5N1금류감병독HA DNA역묘면역BALB/c소서화SPF계.소서화계분별경전천공화기육주사면역량차,간격위3주.이차면역후,용치사량적동원병독진행공독실험.공백대조조재공독후전부사망,이경전천공면역적소서화계균획득료완전적보호,병능유효지억제병독재소서폐장화계설식강적번식.동시,전천공면역적소서화계균산생료고수평적특이성항체.경전천공면역적소서공독후CTL반응명현가강.저사결과표명,HADNA역묘능유효지보호소서화계대금류감병독적감염,동시야표명전천공면역시DNA역묘면역적유효도경지일.
To investigate the protection effect of DNA vaccine in mammalian and avian systems, the DNA vaccine was inoculated in both BALB/c mice and SPF chickens immunized with DNA vaccines encoding hemagglutinin (HA) from A/Goose/GuangDong/1/96 (H5N1) virus. The mice and chickens were immunized twice, 3 weeks apart, by electroporation into muscles or intramuscular injection. Two weeks after the second immunization, the mice and chickens were challenged with a lethal dose of homologous virus. The mice and chickens immunized by electroporation obtained completely protection against the virus, and could effectively inhibited viruses to replicating in mouse lung and chicken cloaca. At the same time, these protections were companied by high levels specific antibody to H5N1 AIV, while the blank plasmid controls experience 100 percent mortality following challenge. Furthermore, in the experiment of mice by eletroporation,stronger obviously CTL activity were observed after challenge. Thus, the cellular immune responses of the mice immunized by electroporation were exhibited. These results strongly demonstrate that HA DNA vaccines provide effective protection against influenza virus infection in mammalian and avian, and suggest that electroporation is one of the efficient gene delivery systems for the transfer of influenza DNA vaccine in both humoral immunity and cellular immunity.