中国临床康复
中國臨床康複
중국림상강복
CHINESE JOURNAL OF CLINICAL REHABILITATION
2006年
39期
186-188,封面
,共4页
陈玉培%牟崇明%季道如%但伶%龚文婷%王莉莎
陳玉培%牟崇明%季道如%但伶%龔文婷%王莉莎
진옥배%모숭명%계도여%단령%공문정%왕리사
参附汤%再灌注损伤%缺血%基因,bcl-2%基因,fos
參附湯%再灌註損傷%缺血%基因,bcl-2%基因,fos
삼부탕%재관주손상%결혈%기인,bcl-2%기인,fos
背景:研究证实,参附注射液对各种休克、心力衰竭、心肌缺血以及室上性/室性心律失常均有改善治疗作用,对大鼠心肌缺血再灌注损伤具有良好的保护作用.目的:观察参附注射液对急性缺血再灌注损伤大鼠心肌凋亡相关基因Bcl-2,Bax和c-Fos蛋白表达的影响.设计:完全随机分组设计,对照实验.单位:重庆医科大学附属第二医院麻醉科.材料:实验于2004-04/12在重庆医科大学附属第二医院麻醉教研室完成.选用健康成年Wistar大鼠35只,由解放军第三军医大学大坪医院实验动物中心提供.参附注射液是中医"回阳救逆"参附汤的中药配方,其主要成分是人参皂甙及乌头类生物碱(雅安三九药业有限公司出品,规格10 mL/支,批号:030110).方法:采用在体心脏缺血再灌注模型,35只大鼠按随机数字表法分为5组,每组7只.①假手术组:只穿线不结扎,静脉注射生理盐水8 mL/kg,观察120 min.②参附注射液30 min组:结扎前15 min静脉注射参附注射液8 mL/kg,结扎左冠状动脉前降支40 min,再灌注30 min.③参附注射液120 min组:再灌注120 min,余同参附注射液30 min组.④生理盐水30 min对照组:结扎左冠状动脉前降支前15 min静脉注射生理盐水8 mL/kg,结扎左冠状动脉前降支40min,再灌注30 mi.⑤生理盐水120min对照组:再灌注120min,余同生理盐水30min组.应用免疫组化法检测各组大鼠心肌凋亡相关基因Bcl-2,Bax和c-Fos蛋白的表达量.主要观察指标:各组大鼠心肌凋亡相关基因Bcl-2,Bax和c-Fos蛋白的表达量.结果:35只大鼠全部进入结果分析,无脱失.①与假手术组比较,生理盐水30 min组和120 min组大鼠心肌Bcl-2,Bax和c-Fos蛋白表达水平显著升高(P＜0.01),Bcl-2/Bax比率显著降低(P＜0.01).②与相应生理盐水组比较,参附注射液30 min组和120 min Bcl-2蛋白表达水平显著升高(P＜0.01),Bax和c-Fos蛋白表达水平显著降低(P＜0.01),Bcl-2/Bax比率显著升高(P＜0.01).结论:参附注射液对缺血再灌注心肌保护效应可能与其促进Bcl-2蛋白高表达、抑制Bax与c-Fos蛋白表达、增加Bcl-2/Bax比率,从而抑制心肌细胞凋亡有关.
揹景:研究證實,參附註射液對各種休剋、心力衰竭、心肌缺血以及室上性/室性心律失常均有改善治療作用,對大鼠心肌缺血再灌註損傷具有良好的保護作用.目的:觀察參附註射液對急性缺血再灌註損傷大鼠心肌凋亡相關基因Bcl-2,Bax和c-Fos蛋白錶達的影響.設計:完全隨機分組設計,對照實驗.單位:重慶醫科大學附屬第二醫院痳醉科.材料:實驗于2004-04/12在重慶醫科大學附屬第二醫院痳醉教研室完成.選用健康成年Wistar大鼠35隻,由解放軍第三軍醫大學大坪醫院實驗動物中心提供.參附註射液是中醫"迴暘救逆"參附湯的中藥配方,其主要成分是人參皂甙及烏頭類生物堿(雅安三九藥業有限公司齣品,規格10 mL/支,批號:030110).方法:採用在體心髒缺血再灌註模型,35隻大鼠按隨機數字錶法分為5組,每組7隻.①假手術組:隻穿線不結扎,靜脈註射生理鹽水8 mL/kg,觀察120 min.②參附註射液30 min組:結扎前15 min靜脈註射參附註射液8 mL/kg,結扎左冠狀動脈前降支40 min,再灌註30 min.③參附註射液120 min組:再灌註120 min,餘同參附註射液30 min組.④生理鹽水30 min對照組:結扎左冠狀動脈前降支前15 min靜脈註射生理鹽水8 mL/kg,結扎左冠狀動脈前降支40min,再灌註30 mi.⑤生理鹽水120min對照組:再灌註120min,餘同生理鹽水30min組.應用免疫組化法檢測各組大鼠心肌凋亡相關基因Bcl-2,Bax和c-Fos蛋白的錶達量.主要觀察指標:各組大鼠心肌凋亡相關基因Bcl-2,Bax和c-Fos蛋白的錶達量.結果:35隻大鼠全部進入結果分析,無脫失.①與假手術組比較,生理鹽水30 min組和120 min組大鼠心肌Bcl-2,Bax和c-Fos蛋白錶達水平顯著升高(P＜0.01),Bcl-2/Bax比率顯著降低(P＜0.01).②與相應生理鹽水組比較,參附註射液30 min組和120 min Bcl-2蛋白錶達水平顯著升高(P＜0.01),Bax和c-Fos蛋白錶達水平顯著降低(P＜0.01),Bcl-2/Bax比率顯著升高(P＜0.01).結論:參附註射液對缺血再灌註心肌保護效應可能與其促進Bcl-2蛋白高錶達、抑製Bax與c-Fos蛋白錶達、增加Bcl-2/Bax比率,從而抑製心肌細胞凋亡有關.
배경:연구증실,삼부주사액대각충휴극、심력쇠갈、심기결혈이급실상성/실성심률실상균유개선치료작용,대대서심기결혈재관주손상구유량호적보호작용.목적:관찰삼부주사액대급성결혈재관주손상대서심기조망상관기인Bcl-2,Bax화c-Fos단백표체적영향.설계:완전수궤분조설계,대조실험.단위:중경의과대학부속제이의원마취과.재료:실험우2004-04/12재중경의과대학부속제이의원마취교연실완성.선용건강성년Wistar대서35지,유해방군제삼군의대학대평의원실험동물중심제공.삼부주사액시중의"회양구역"삼부탕적중약배방,기주요성분시인삼조대급오두류생물감(아안삼구약업유한공사출품,규격10 mL/지,비호:030110).방법:채용재체심장결혈재관주모형,35지대서안수궤수자표법분위5조,매조7지.①가수술조:지천선불결찰,정맥주사생리염수8 mL/kg,관찰120 min.②삼부주사액30 min조:결찰전15 min정맥주사삼부주사액8 mL/kg,결찰좌관상동맥전강지40 min,재관주30 min.③삼부주사액120 min조:재관주120 min,여동삼부주사액30 min조.④생리염수30 min대조조:결찰좌관상동맥전강지전15 min정맥주사생리염수8 mL/kg,결찰좌관상동맥전강지40min,재관주30 mi.⑤생리염수120min대조조:재관주120min,여동생리염수30min조.응용면역조화법검측각조대서심기조망상관기인Bcl-2,Bax화c-Fos단백적표체량.주요관찰지표:각조대서심기조망상관기인Bcl-2,Bax화c-Fos단백적표체량.결과:35지대서전부진입결과분석,무탈실.①여가수술조비교,생리염수30 min조화120 min조대서심기Bcl-2,Bax화c-Fos단백표체수평현저승고(P＜0.01),Bcl-2/Bax비솔현저강저(P＜0.01).②여상응생리염수조비교,삼부주사액30 min조화120 min Bcl-2단백표체수평현저승고(P＜0.01),Bax화c-Fos단백표체수평현저강저(P＜0.01),Bcl-2/Bax비솔현저승고(P＜0.01).결론:삼부주사액대결혈재관주심기보호효응가능여기촉진Bcl-2단백고표체、억제Bax여c-Fos단백표체、증가Bcl-2/Bax비솔,종이억제심기세포조망유관.
BACKGROUND: It has been confirmed that Shenfu parenteral injection can ameliorate and treat various shocks, heart failure, myocardial ischemia and supraventricular/ventricular arrhythmia, and it also has a good protective effect on myocardial ischemia/reperfusion injury in rats.OBJECTIVE: To observe the effects of Shenfu parenteral injection on the protein expressions of myocardial apoptosis-related genes of Bcl-2, Bax and c-Fos in rats with acute ischemia/reperfusion injury.DESIGN: A complete randomized grouping design, controlled experiment.SETTING: Department of Anesthesiology, the Second Affiliated Hospital,Chongqing University of Medical Sciences.MATERIALS: The experiments were carried out in the Staff Room of Anesthesiology, the Second Affiliated Hospital, Chongqing University of Medical Sciences from April to December in 2004. Thirty-five healthy adult Wistar rats were provided by the experimental animaI center of Daping Hospital, Third Military Medical University of Chinese PLA. Shenfu parenteral injection was the TCM formula of Shenfu Tang, which is for recuperating depleted yang and rescuing the patient from collapse, and its main components are ginsenoside and aconitum alkaloid. It was the product of Yaan Sanjiu Pharmaceutical Co., Ltd., 10 mL/piece, the batch number was 030110.METHODS: In vivo models of myocardial ischemia/reperfusion injury were used. The 35 rats were divided into 5 groups according to the number of random number table, with 7 rats in each group: ① Sham-operated group: The rats were treated with only insertion of thread without ligation, followed by intravenous injection of saline (8 mL/kg), and then observed for 120 minutes. ② Shenfu parenteral injection 30-minute group: The rats were treated with intravenous injection of Shenfu parenteral injection (8 mL/kg) at 15 minutes before ligation, and then the left coronary artery anterior descending branch was ligated for 40 minutes and reperfused for 30 minutes. ③ Shenfu parenteral injection 120-minute group: The rats were reperfused for 120 minutes, and the others were the same as those in the Shenfu parenteral injection 30-minute group. ④ Saline 30-minute control group: The rats were treated with intravenous injection of saline (8 mL/kg) at 15 minutes before ligation, and then the left coronary artery anterior descending branch was ligated for 40 minutes and reperfused for 30 minutes. ⑤ Saline 120-minute control group: The rats were reperfused for 120 minutes, and the others were the same as those in the saline 30-minute control group. The expressions of Bcl-2, Bax and c-Fos proteins in myocardial tissues were detected with immunohistochemical method.MAIN OUTCOME MEASURES: The expressions of Bcl-2, Bax and cFos proteins in myocardial tissues of rats in each group were observed.RESULTS: All the 35 rats were involved in the analysis of results without deletion. ①As compared with the sham-operated group, the expressions of Bcl-2, Bax or c-Fos protein in myocardium were significantly increased(P ＜ 0.01), but the Bcl-2/Bax ratios were significantly decreased (P ＜0.01) in the saline 30 and 120-minute groups. ② As compared with corresponding saline groups, the expressions of Bcl-2 protein in the Shenfu parenteral injection 30 and 120-minute groups were significantly increased (P ＜ 0.01), the expressions of Bax and c-Fos proteins were remarkably decreased (P ＜ 0.01), and the Bcl-2/Bax ratios were significantly increased (P ＜ 0.01).CONCLUSION: Protective effect of Shenfu parenteral injection on ischemia/reperfusion myocardium may be correlated with its promotion of Bcl-2 protein expression, restrain to Bax and c-Fos protein expressions, and increase of Bcl-2/Bax ratio, and it restrains the apoptosis of myocardial cells.
