CAJ | 학술논문

目的研究掌叶半夏超临界CO2乙醇萃取物(SEE-CO2PP)对青霉素诱发惊厥的对抗作用.方法采用大鼠皮质局部定位注射青霉素诱发惊厥模型,研究SEE-CO2PP对惊厥发作的潜伏期以及惊厥行为变化的影响,并用RM6240C型多道生理信号采集处理仪记录皮质和海马痫性放电的潜伏期、频率和痫波最高发放波幅,同时应用高效液相色谱法测定海马谷氨酸(Glu)、天冬氨酸 (Asp)、甘氨酸(Gly)和γ-氨基丁酸(GABA)递质的含量.结果 SEE-CO2PP 15和30 g·kg-1 (ig)可延长青霉素诱发惊厥的潜伏期,并减弱发作强度.SEE-CO2PP能够延长痫性放电的潜伏期,减少痫性放电的频率,减小皮质和海马发放痫波的最高波幅,同时,SEE-CO2PP可以增加海马GABA的水平,对Gly,Asp和Glu水平无明显影响.结论 SEE-CO2PP可对抗青霉素诱发的惊厥行为和痫样放电,具有抗惊厥作用.
목적 연구장협반하초림계CO2을순췌취물(SEE-CO2PP)대청매소유발량궐적대항작용.방법 채용대서피질국부정위주사청매소유발량궐모형,연구SEE-CO2PP대량궐발작적잠복기이급량궐행위변화적영향,병용RM6240C형다도생리신호채집처리의기록피질화해마간성방전적잠복기、빈솔화간파최고발방파폭,동시응용고효액상색보법측정해마곡안산(Glu)、천동안산 (Asp)、감안산(Gly)화γ-안기정산(GABA)체질적함량.결과 SEE-CO2PP 15화30 g·kg-1 (ig)가연장청매소유발량궐적잠복기,병감약발작강도.SEE-CO2PP능구연장간성방전적잠복기,감소간성방전적빈솔,감소피질화해마발방간파적최고파폭,동시,SEE-CO2PP가이증가해마GABA적수평,대Gly,Asp화Glu수평무명현영향.결론 SEE-CO2PP가대항청매소유발적량궐행위화간양방전,구유항량궐작용.
AIM To investigate the anticonvulsive action of supercritical CO2 ethanol extract from Pinellia Pedatisecta Schott(SEE-CO2PP). METHODS The rat convulsive model was induced by penicillin localized injected in rat cortex. The effects of SEE-CO2PP on the latency of seizure and changes of convulsive behaviors were investigated. The latency of epileptiform discharge, and frequency and amplitude of highest wave in cortex and hippocampus were recorded by using RM6240C multichannel physiological signal collection and analysis recorder. At the same time, the contents of glutamic acid (Glu), aspartic acid (Asp), glycine (Gly) and γ-aminobutyric acid (GABA) in hippocampus were determined with high performance liquid chromatography. RESULTS SEE-CO2PP 15 and 30 g·kg-1, ig, prolonged the latent period of seizure and weakened the extent. SEE-CO2PP also prolonged the latent period of epileptiform discharge, reduced the frequency and decreased amplitude of the highest wave in both cortex and hippocampus. Moreover, SEE-CO2PP increased the content of GABA in hippocampus, but the levels of Gly,Asp and Glu had no obvious changes. CONCLUSION SEE-CO2PP inhibits the epileptiform discharge and convulsive behaviors of convulsive model rats, which suggests SEE-CO2PP has anticonvulsive action.