CAJ | 학술논문

待测样品以适量1%三氯乙酸溶液提取,2%乙酸铅溶液沉淀蛋白,离心后上清液经0.45 μm滤膜过滤后直接进行HPLC分析.据此建立了蛋白类保健食品中非法添加三聚氰胺的高效液相色谱-二级管阵列检测新方法.方法的线性范围为0.02～2.0 mg/L,r =0.999 5,检出限为0.14 ng.该方法简便、快速,灵敏度高,可准确排除阴性干扰,易推广应用.
대측양품이괄량1%삼록을산용액제취,2%을산연용액침정단백,리심후상청액경0.45 μm려막과려후직접진행HPLC분석.거차건립료단백류보건식품중비법첨가삼취청알적고효액상색보-이급관진렬검측신방법.방법적선성범위위0.02～2.0 mg/L,r =0.999 5,검출한위0.14 ng.해방법간편、쾌속,령민도고,가준학배제음성간우,역추엄응용.
The sample was extracted by 1% TCA solution,and 2% lead acetate solution for protein precipitation.As centrifuged,supernatant layer was filtrated with 0.45 μm membrane for HPLC analysis.The linear range for the method was 0.02～2.0 mg/L,r=0.999 5,and detection limit for the method was 0.14 ng.The method is applicable for the determination of melamine in nitrogenous health food products.Spectrum of diode array detector is easy to exclude negative interference.Results show that the method is simple,rapid,sensitive and easy to be applied.