CAJ | 학술논문

建立猪肉中类固醇类激素残留高效液相色谱-串联质谱(LC-MS/MS)分析方法.样品经甲醇提取,同相萃取柱净化后采用LC-MS/MS进行检测分析;并对样品前处理条件和质谱参数进行研究.结果表明:这些激素物质的线性范围均为0.5～100 μg/kg;低、中、高3种加标水甲的加标回收率为71%～89%;最低榆出限为0.2～2 μg/kg.本方法快速简便、灵敏度高、选择性好、测定结果令人满意,可在各食品柃测机构推广应用.
건립저육중류고순류격소잔류고효액상색보-천련질보(LC-MS/MS)분석방법.양품경갑순제취,동상췌취주정화후채용LC-MS/MS진행검측분석;병대양품전처리조건화질보삼수진행연구.결과표명:저사격소물질적선성범위균위0.5～100 μg/kg;저、중、고3충가표수갑적가표회수솔위71%～89%;최저유출한위0.2～2 μg/kg.본방법쾌속간편、령민도고、선택성호、측정결과령인만의,가재각식품령측궤구추엄응용.
A method using high-performance liquid chromatography coupled with tandem mass spectrometry(HPLC-MS/MS)was proposed for the determination of steroid hormone multi-residues in pork.Analytes were extracted from samples with methanol and cleaned up using a solid-phase extraction cartridge prior to HPLC-MS/MS analysis.The proposed method exhibited a good linearity for 10 steroid hormones over the range of 0.5-100 μg/kg and a limit of detection ranging from 0.2 to 2 μg/kg.Mean recoveries for 10 steroid hormones in blank pork samples spiked at low,medium and high concentrations were between 71% and 88%,with relative standard deviations below 10%.The method is characteristic of rapidity,simplicity,good selectivity and high sensitivity,and therefore has a great popularization potential.