CAJ | 학술논문

目的探讨金丝桃素被光激活后对C6胶质瘤的抑制作用及其对血管细胞黏附因子-1(VCAM-1)和基质金属蛋白酶-9(MMP-9)的影响.方法 36只C6脑内胶质瘤模型Wistar大鼠随机分为6组:对照组,单纯光照组,金丝桃素低、高剂量非光照组,金丝桃素低、高剂量光照组.实验第2周和3周MRI观察肿瘤的生长情况.第3周切取肿瘤,称重计算抑瘤率.SP法检测瘤组织中VCAM-1和MMP-9.结果 (1)MRI检测显示,金丝桃素光照组肿瘤体积比对照组明显减小(P<0.05).(2)高剂量光照组抑瘤率为75.6%;低剂量光照组抑瘤率为51.4%.单纯光照组、金丝桃素非光照组对肿瘤无抑制作用.(3)金丝桃素光照组中VCAM-1和MMP-9明显低于对照组(P<0.05).VCAM-1与MMP-9呈正相关(r=0.82).结论光激活的金丝桃素可能通过抑制VCAM-1和MMP-9,降低肿瘤突破基质屏障的能力,抑制肿瘤新生血管形成,而制约胶质瘤生长和侵袭.
목적 탐토금사도소피광격활후대C6효질류적억제작용급기대혈관세포점부인자-1(VCAM-1)화기질금속단백매-9(MMP-9)적영향.방법 36지C6뇌내효질류모형Wistar대서수궤분위6조:대조조,단순광조조,금사도소저、고제량비광조조,금사도소저、고제량광조조.실험제2주화3주MRI관찰종류적생장정황.제3주절취종류,칭중계산억류솔.SP법검측류조직중VCAM-1화MMP-9.결과 (1)MRI검측현시,금사도소광조조종류체적비대조조명현감소(P<0.05).(2)고제량광조조억류솔위75.6%;저제량광조조억류솔위51.4%.단순광조조、금사도소비광조조대종류무억제작용.(3)금사도소광조조중VCAM-1화MMP-9명현저우대조조(P<0.05).VCAM-1여MMP-9정정상관(r=0.82).결론 광격활적금사도소가능통과억제VCAM-1화MMP-9,강저종류돌파기질병장적능력,억제종류신생혈관형성,이제약효질류생장화침습.
Objective To study of therapeutic effect and mechanism of photo-activated hypericin on rat C6 glioma. Methods At first, C6 glioma auto-transplanted animal models were established, then using different dose of hypericin to act on the models under light or without light.After 3 weeks, tumors were removed and dealed with immunohistochemistry. Finally, using statistic methods to analyze the results. Results (1) The tumor weight in hypericin + light group was significantly lower than that in the control. (2) The effect of hypericin showed a trait of dosage dependence in inhibiting the growth of glioma under light, and hypericin. It inhibited 75.6% of tumors in the high dose hypeficin + light group, on the other hand, it inhibited 51.4 % of the tumors in the low dose hypericin + light group. There was no inhibited effect on tumors in the light or hypericin groups. (3) The expression of VCAM-1 and MMP-9 was much lower in the hypericin + light groups than that of the control blank group. And there was a positive relationship between VCAM-1 and MMP-9 (r=0.82).Conclusions This experiment shows, hypericin can effectively inhibit the growth of C6 glioma under light; hypericin + light signifi-cantly refrains the expression of VCAM-1 and MMP-9. So we think photo-activated hypericin could inhibit VEGF receptor and tumor's angiogenesis. This is probably one of the most important mechanisms of inhibiting glioma.