CAJ | 학술논문

目的探讨caspase-10表达在甲状腺分化癌(DTC)发生、发展及预后中的作用.方法 37例DTC来自于2006年1月至2007年12月行甲状腺切除术患者.取DTC患者病理标本,链霉菌抗生物素蛋白-过氧化物酶连结法(S-P)染色,光镜下检测DTC患者caspase-10表达,分析caspase-10在DTC中的表达与临床病理特征之间的关系.取同期46例结节性甲状腺肿组织标本作为对照.结果结节性甲状腺肿细胞质或细胞膜,可见到较多的棕色或黄色caspase-10表达颗粒,但在DTC中棕色或黄色表达颗粒不明显.caspase-10表达阳性率在DTC患者中为29.73%(11/37),在结节性甲状腺肿患者中为71.74%(33/46),二者比较差异有统计学意义(χ2=14528,P<0.01);在DTC有淋巴结转移患者中,caspase-10阳性表达率(11.11%,2/18)明显低于无淋巴结转移患者(47.37%,9/19),二者比较差异有统计学意义(χ2=4.210,P<0.01);caspase-10表达与性别、年龄、国际肿瘤(TNM)分期以及病理分型无关(P>0.05).结论 caspase-10表达下调在甲状腺分化癌的发生和侵袭中发挥重要作用.
목적 탐토caspase-10표체재갑상선분화암(DTC)발생、발전급예후중적작용.방법 37례DTC래자우2006년1월지2007년12월행갑상선절제술환자.취DTC환자병리표본,련매균항생물소단백-과양화물매련결법(S-P)염색,광경하검측DTC환자caspase-10표체,분석caspase-10재DTC중적표체여림상병리특정지간적관계.취동기46례결절성갑상선종조직표본작위대조.결과 결절성갑상선종세포질혹세포막,가견도교다적종색혹황색caspase-10표체과립,단재DTC중종색혹황색표체과립불명현.caspase-10표체양성솔재DTC환자중위29.73%(11/37),재결절성갑상선종환자중위71.74%(33/46),이자비교차이유통계학의의(χ2=14528,P<0.01);재DTC유림파결전이환자중,caspase-10양성표체솔(11.11%,2/18)명현저우무림파결전이환자(47.37%,9/19),이자비교차이유통계학의의(χ2=4.210,P<0.01);caspase-10표체여성별、년령、국제종류(TNM)분기이급병리분형무관(P>0.05).결론 caspase-10표체하조재갑상선분화암적발생화침습중발휘중요작용.
Objective To investigate the expression of caspase-10 in differentiated thyroid carcinoma and association with its development and metastasis. Methods Thyroid samples from 37 patients in a period from January 2006 to December 2007, with differentiated thyroid carcinoma were retrospectively analyzed for caspase-10 by immunohistocbemistry(streptavidin-perosidase, S-P), compared to control group of 46 cases with nodtdar goiter. The relationship between the expression of caspase-10 and the clinical pathologic characteristics of thyroid carcinoma were also explored simultaneously. Results caspase-10 were observed as brown or yellow particles located in the cytoplasm or cell membrane of nodular goiter but there were no significant evidence for its positive expression in thyroid carcinoma, caspase-10 expression was markedly down-regulated in differentiated thyroid carcinoma(29.73%,11/37) compared with benign nodules(71.74%,33/46, χ2=14.528, P<0.01). The positive expression in 18 cases with lymph node metastasis(11.11%,2/18) was significantly lower than those in 19 patients without lymph node metastasis(47.37%,9/19; χ2=4.210, P<0.01). There was no significant correlation(P> 0.05) between the expression of caspase-10 and the clinical pathologic characteristics including male, age, TNM stage and pathologic type. Conclusion Down-regulation of caspase-10 may play a critical role in carcinogenesis and development of differentiated thyroid carcinoma.