中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2010年
28期
32-34
,共3页
紫癜,血小板减少性,特发性%淋巴细胞亚群%抗体
紫癜,血小闆減少性,特髮性%淋巴細胞亞群%抗體
자전,혈소판감소성,특발성%림파세포아군%항체
Purpura,thrombocytopenic,idiopathic%Lymphocyte subsets%Antibodies
目的 检测特发性血小板减少性紫癜(ITP)患者外周血淋巴细胞亚群、血小板膜糖蛋白(GPⅡb/Ⅲa、GPⅠb/Ⅸ)特异性抗体变化,探讨相关因素在ITP发病机制中的作用.方法 用流式细胞术检测72例ITP患者(ITP组)和50例健康体检者(对照组)外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+、CD19+变化.应用改良血小板抗原单克隆抗体固相化检测技术测定GPⅡb/Ⅲa、GPⅠb/Ⅸ特异性抗体变化.结果 ITP组与对照组比较,GPⅡb/Ⅲa、GP Ⅰ b/Ⅸ特异性抗体吸光度值升高(P＜0.05),CD3+、CD4+、CD4+/CD8+显著下降(P＜0.01),CD8+、CD19+显著增高(P＜0.01).结论 淋巴细胞亚群及血小板特异性抗体可较好地反映ITP的病理机制,在ITP的发病机制中起非常重要的作用.
目的 檢測特髮性血小闆減少性紫癜(ITP)患者外週血淋巴細胞亞群、血小闆膜糖蛋白(GPⅡb/Ⅲa、GPⅠb/Ⅸ)特異性抗體變化,探討相關因素在ITP髮病機製中的作用.方法 用流式細胞術檢測72例ITP患者(ITP組)和50例健康體檢者(對照組)外週血淋巴細胞亞群CD3+、CD4+、CD8+、CD4+/CD8+、CD19+變化.應用改良血小闆抗原單剋隆抗體固相化檢測技術測定GPⅡb/Ⅲa、GPⅠb/Ⅸ特異性抗體變化.結果 ITP組與對照組比較,GPⅡb/Ⅲa、GP Ⅰ b/Ⅸ特異性抗體吸光度值升高(P＜0.05),CD3+、CD4+、CD4+/CD8+顯著下降(P＜0.01),CD8+、CD19+顯著增高(P＜0.01).結論 淋巴細胞亞群及血小闆特異性抗體可較好地反映ITP的病理機製,在ITP的髮病機製中起非常重要的作用.
목적 검측특발성혈소판감소성자전(ITP)환자외주혈림파세포아군、혈소판막당단백(GPⅡb/Ⅲa、GPⅠb/Ⅸ)특이성항체변화,탐토상관인소재ITP발병궤제중적작용.방법 용류식세포술검측72례ITP환자(ITP조)화50례건강체검자(대조조)외주혈림파세포아군CD3+、CD4+、CD8+、CD4+/CD8+、CD19+변화.응용개량혈소판항원단극륭항체고상화검측기술측정GPⅡb/Ⅲa、GPⅠb/Ⅸ특이성항체변화.결과 ITP조여대조조비교,GPⅡb/Ⅲa、GP Ⅰ b/Ⅸ특이성항체흡광도치승고(P＜0.05),CD3+、CD4+、CD4+/CD8+현저하강(P＜0.01),CD8+、CD19+현저증고(P＜0.01).결론 림파세포아군급혈소판특이성항체가교호지반영ITP적병리궤제,재ITP적발병궤제중기비상중요적작용.
Objective To test the changes of lymphocyte subsets and anti-GP Ⅱ b/Ⅲa,anti-GP Ⅰ b/Ⅸ specific antibodies in patients with idiopathic thrombocytopenic purpura(ITP),and discuss the related factors involved in pathogenesis of ITP.Methods The changes of lymphocyte subsets CD3+,CD4+,CD8+,CD4+CD8+,CD19+ in 72 patients with ITP (ITP group) and 50 healthy controls (control group) were measured by flow cytometry (FCM).Expression of anti-GP Ⅱ b/Ⅲ a,anti-GP Ⅰ b/Ⅸ specific antibodies were tested by monoclonal antibody immobilization of platelet antigens assay (modified MAIPA).Results Optical density of anti-GP Ⅱ b/Ⅲ a,anti-GP Ⅰ b/Ⅸ specific antibodies in ITP group was much higher than that in control group (P ＜0.05).In changes of lymphocyte subsets,the number of the CD3+,CD4+,CD4+/CD8+ in ITP group were obviously lower than those in control group (P ＜0.01),but CD8+ and CD19+ were much higher than those in control group(P ＜0.01).Conclusions Expression of lymphocyte subsets and platelet specific antibodies can reflect the pathogenesis of ITP well.The abnormal function and ratio of lymphocyte subsets,as well as the immunologic mechanism disorder of lymphocytes play important roles in the pathogenesis of ITP.
