CAJ | 학술논문

目的检测单核巨噬细胞抗原(ED)-1在大鼠局灶节段肾小球硬化(FSCS)中的表达,探讨单核巨噬细胞浸润在FSGS发生发展中的作用.方法 12只雄性Wisuw大鼠,实验组7只,一次性静脉注射氨基核苷嘌呤霉素9mg/100 g体重,对照组5只,静脉注射生理盐水一次,动态观察尿蛋白定量变化.第20周末宰杀,检测血浆白蛋白、血脂、肾功能.肾脏制备病理标本.应用免疫组织化学法观察两组大鼠肾组织中纤维连接蛋白(FN)和ED1阳性单核巨噬细胞的表达,计数ED1阳性细胞数.分析其与尿蛋白程度及FN表达的相关性.结果 FSGS模型组大鼠用药后尿蛋白定量显著增高,血总胆固醇显著增高.肾脏病理示部分肾小球局灶节段硬化或全球性硬化.细胞外基质节段性增多.在模型组大鼠肾组织中ED1阳性细胞较正常大鼠肾组织显著增多(P<0.01).模型组大鼠肾组织中FN的表达较正常大鼠肾组织明显增多(P<0.05).ED1阳性细胞数与FN表达呈直线正相关(r=0.876,P<0.01).ED1阳性细胞数与尿蛋白程度呈直线正相关(r=0.651,P<0.01).结论肾间质单核巨噬细胞浸润可以加重细胞外基质沉积,加重肾小球硬化,提示炎症反应在局灶节段肾小球硬化的发生与发展中起一定作用.
목적 검측단핵거서세포항원(ED)-1재대서국조절단신소구경화(FSCS)중적표체,탐토단핵거서세포침윤재FSGS발생발전중적작용.방법 12지웅성Wisuw대서,실험조7지,일차성정맥주사안기핵감표령매소9mg/100 g체중,대조조5지,정맥주사생리염수일차,동태관찰뇨단백정량변화.제20주말재살,검측혈장백단백、혈지、신공능.신장제비병리표본.응용면역조직화학법관찰량조대서신조직중섬유련접단백(FN)화ED1양성단핵거서세포적표체,계수ED1양성세포수.분석기여뇨단백정도급FN표체적상관성.결과 FSGS모형조대서용약후뇨단백정량현저증고,혈총담고순현저증고.신장병리시부분신소구국조절단경화혹전구성경화.세포외기질절단성증다.재모형조대서신조직중ED1양성세포교정상대서신조직현저증다(P<0.01).모형조대서신조직중FN적표체교정상대서신조직명현증다(P<0.05).ED1양성세포수여FN표체정직선정상관(r=0.876,P<0.01).ED1양성세포수여뇨단백정도정직선정상관(r=0.651,P<0.01).결론 신간질단핵거서세포침윤가이가중세포외기질침적,가중신소구경화,제시염증반응재국조절단신소구경화적발생여발전중기일정작용.
Objective To assay the expression chinse of ED1 positive cell in focal segmental glomemlosclerosis in rats,we investigated the relationship between the infiltration of mononuclear phagocytes and the progression of glomendar sclerosis.Methods We used 12 Wistar ratswhichwere dividedintotwo groups.1est group and coutrol group.Themodel offocal segmental glomerursclerosiswas ulade by in jecting PAN 9 mg/100g body weights.The rats ofcontrol group were injected 3ml 0.9％80diuln chloride.The proteinuria,serum creatinine,fipi&and protein of the rats were examined.The rata were killed at the 20th week.All the kidneys were kept and nlade into pathologic slEun-pie.1mmunohistochemical method was applied to detect the protein expression of FIN and the EDI positive cell in renal tissue of all the rats,and the number of Edl positive cell W88 counted.The results were analyzed by SPSS.Results The proteinuria of the mts in FSGS model group was significantly increased,the serum lipid ofthem was also increased.The pathology changes of the rat renal in model group showed that a part of giomemli appeared focal segmental sclerosis or all glomerular sclerosis,and the extracelhlar matrix accumulated.In the renal of model rats,the amount of EDI positive cells was significantly higher than that in normal rats(P<O.05).The expression of FN in the rehal of model rats was significantly higher than that in the normal rats(P<0.05).111e mount ofEDl positive cells WaS significantly positively correlated WitII the expression of FN(P=0.002).The amount of Edl positive cells W88 significantly positively correlated with the pmteinuria of the rats(P=0.014).Conclusion Theinfiltration ofEDI positivecell contributed to the progression of glomerular sclerosis.