CAJ | 학술논문

为研究胆总管狭窄时肝组织BR-UDPGT基因表达的改变.本研究用雄性Wistar大鼠不完全结扎胆总管造成胆总管狭窄动物模型.采用Northern印迹杂交和测定BR-UDPGT活性的方法,研究胆总管狭窄对肝组织BR-UDPGT基因表达的影响.结果显示:在胆总管狭窄后12 h～14 d的不同时间段,肝组织BR-UDPGT在mRNA水平均未见基因表达较正常对照组降低.但BR-UDPGT酶活性在胆总管狭窄后12 h后即开始下降,且随狭窄时间的延长BR-UDPGT活性逐渐下降.胆总管狭窄确可引起肝组织BR-UDPGT的活性下降.作用机制不在BR-UDPGT的基因转录水平,可能在转录后的其他环节上.BR-UDPGT活性下降可能是胆总管狭窄后胆汁中的UCB浓度增高的原因之一.
위연구담총관협착시간조직BR-UDPGT기인표체적개변.본연구용웅성Wistar대서불완전결찰담총관조성담총관협착동물모형.채용Northern인적잡교화측정BR-UDPGT활성적방법,연구담총관협착대간조직BR-UDPGT기인표체적영향.결과현시:재담총관협착후12 h～14 d적불동시간단,간조직BR-UDPGT재mRNA수평균미견기인표체교정상대조조강저.단BR-UDPGT매활성재담총관협착후12 h후즉개시하강,차수협착시간적연장BR-UDPGT활성축점하강.담총관협착학가인기간조직BR-UDPGT적활성하강.작용궤제불재BR-UDPGT적기인전록수평,가능재전록후적기타배절상.BR-UDPGT활성하강가능시담총관협착후담즙중적UCB농도증고적원인지일.
This essay is aimed to study the change of the expression of BR-UDPGT. The elevated concentration of unconjugated bilirubin (UCB) in bile plays a crucial role in the pathogenesis of PS. It is well known that the bile duct stricture is one of the factors related to pathogenesis of PS. In the PS animal model induced by common bile duct (CBD) stricture, neither biliary infection nor enhanced activity of β-glucuronidase (β-G) were identified, whereas the concentration of UCB in bile was markedly elevated and PS formed.Therefore, in order to further study the pathogenesis of PS, it is necessary to investigate the change of BRUDPGT after CBD stricture. Adult male Wistar rats were divided into control and several CBD stricture groups. The expression of BR-UDPGT was investigated by Northern blot and measuring the activity of BRUDPGT. During the period of 12 hours to 14 days after CBD stricture, the expression of BR-UDPGT in mRNA was almost the same as that of the control group. But the activity of BR-UDPGT was reduced 12 hours after CBD stricture, from 14. 82 μmol/g (protein) /h ± 0. 45 μmol/g (protein) /h to 11.67 μmol/g (protein) /h ± 1.26μmol/g (protein)/h, ( -x ± s, both n = 5, P ＜0. 01). And it became much lower with the duration of CBD stricture. CBD stricture decreased the activity of BR-UDPGT and reduced the ability of liver tissues to transfer UCB into CB. The level affected by CBD stricture was not the transcription of BR-UDPGT. Other steps after gene transcription may be involved. The decreased activity of BR-UDPGT may be one of the causes of elevated UCB concentration after CBD stricture.