CAJ | 학술논문

目的检测热激对Jurkat细胞中主要组织相容性复合物Ⅱ类转录激活因子(CⅡTA)的表达,及其对下游人类白细胞抗原(HLA-DR)表达的影响.方法采用实时RT-PCR检测42℃热激和干扰素-γ(IFN-γ)作用前后Jurkat细胞中CⅡTA mRNA的变化.采用Western blot检测热激和IFN-γ作用前后Jurkat细胞中CⅡTA 蛋白的表达变化.采用流式细胞仪荧光分析热激前后Jurkat细胞表面HLA-DR的表达.结果在Jurkat细胞中,热激可诱导CⅡTA mRNA和蛋白的表达,而IFN-γ处理后无明显变化.与正常Jurkat细胞相比,热激后HLA-DR在Jurkat细胞表面的抗原浓度明显增加(P<0.01).结论热诱导Jurkat细胞中CⅡTA和HLA-DR先后表达增高.提示热激可能在免疫基因表达缺陷的细胞中,重建相关基因的功能,为肿瘤热疗提供新的依据.
목적 검측열격대Jurkat세포중주요조직상용성복합물Ⅱ류전록격활인자(CⅡTA)적표체,급기대하유인류백세포항원(HLA-DR)표체적영향.방법 채용실시RT-PCR검측42℃열격화간우소-γ(IFN-γ)작용전후Jurkat세포중CⅡTA mRNA적변화.채용Western blot검측열격화IFN-γ작용전후Jurkat세포중CⅡTA 단백적표체변화.채용류식세포의형광분석열격전후Jurkat세포표면HLA-DR적표체.결과 재Jurkat세포중,열격가유도CⅡTA mRNA화단백적표체,이IFN-γ처리후무명현변화.여정상Jurkat세포상비,열격후HLA-DR재Jurkat세포표면적항원농도명현증가(P<0.01).결론 열유도Jurkat세포중CⅡTA화HLA-DR선후표체증고.제시열격가능재면역기인표체결함적세포중,중건상관기인적공능,위종류열료제공신적의거.
Objective To explore the effect of a non-lethal heat shock, in comparison with the treatment of interferon-gamma (IFNγ), on the expression of major histocompatibility complex Ⅱ transactivator (CⅡTA) and its downstream target gene of the human leukocyte antigens (HLA)-DR in Jurkat cells. MethodsThe changes of CⅡTA mRNA in Jurkat cells before and after the treatment of heat shock or IFNγ were detected using real time RT-PCR. The changes of CⅡTA protein were detected with Western blot. The expression of HLA-DR was detected with flow cytometry. ResultsCⅡTA mRNA and protein were induced in Jurkat cells under heat shock, but not with IFN-γ. The expression of HLA-DR gene significantly increased after recovery (P<0.01). ConclusionThe expressions of CⅡTA and HLA-DR in Jurkat cells remarkably increase after heat shock, indicating that heat shock may help reconstruct relevant genes in cells with immunologic gene deficiencies.