生物技术通报
生物技術通報
생물기술통보
BIOTECHNOLOGY BULLETIN
2010年
3期
6-12
,共7页
沈宝明%谭新球%谭周进%刘勇
瀋寶明%譚新毬%譚週進%劉勇
침보명%담신구%담주진%류용
工程菌%目的基因%高效表达%影响因素
工程菌%目的基因%高效錶達%影響因素
공정균%목적기인%고효표체%영향인소
Engineering bacteria%Target genes%High-level expression%Influencing factors
目的基因在细胞中的表达研究,在理论和应用上都具有重要的意义.只有弄清影响目的基因表达的因素,才能使目的基因高效表达.主要讨论目的基因本身、表达载体、宿主细胞、培养条件、诱导剂等因素对目的基因更加高效的表达的影响.
目的基因在細胞中的錶達研究,在理論和應用上都具有重要的意義.隻有弄清影響目的基因錶達的因素,纔能使目的基因高效錶達.主要討論目的基因本身、錶達載體、宿主細胞、培養條件、誘導劑等因素對目的基因更加高效的錶達的影響.
목적기인재세포중적표체연구,재이론화응용상도구유중요적의의.지유롱청영향목적기인표체적인소,재능사목적기인고효표체.주요토론목적기인본신、표체재체、숙주세포、배양조건、유도제등인소대목적기인경가고효적표체적영향.
Many complex influencing factors were paid growing emphasis on making target genes high-level expression in host cells in the procedure of gene expression.Mainly influence factors including target genes,expression vectors,host cells,culture conditions,and inducers were discussed in detail in this paper.To handle them efficiently is useful and necessary to have target genes express as anticipated in theory and in practice