CAJ | 학술논문

背景:有研究报道,在包括血管内皮生长因子在内的多种因子诱导下,单核细胞能够转化成血管内皮细胞;而在体外条件下,单核细胞能否转化成淋巴管内皮细胞,至今未见报道.目的:观察单核细胞存炎症环境中转分化成淋巴管内皮细胞的可能性.方法:采用Ficoll密度梯度离心法分离成人新鲜外周血单核细胞,用细胞培养基培养,然后分别用纤维连接蛋白、肿瘤坏死因子α诱导24 h.用RT-PCR和免疫细胞化学方法检测单核细胞对淋巴管内皮特异性标志物LYVE-1、Podoplanin、Prox1、血管内皮生长因子受体3以及内皮细胞抗原vWF、内皮型一氧化氮合酶和血管内皮生长因子受体2的摹因和蛋白表达.结果与结论:单核细胞在诱导之前,对LYVE-1表达阳性,对Podoplanin、Prox1、血管内皮生长因子受体3、vWF、内皮型一氧化氮合酶和血管内皮生长因子受体2表达均为阴性;经上述因子刺激后,单核细胞对Podoplanin、Prox-1、血管内皮生长因子受体3表达阳性,vWF、内皮型一氧化氮合酶和血管内皮生长因子受体2表达仍呈阴性.提示纤维连接蛋白、肿瘤坏死因子α均能有效刺激单核细胞表达淋巴管内皮标志物.
배경:유연구보도,재포괄혈관내피생장인자재내적다충인자유도하,단핵세포능구전화성혈관내피세포;이재체외조건하,단핵세포능부전화성림파관내피세포,지금미견보도.목적:관찰단핵세포존염증배경중전분화성림파관내피세포적가능성.방법:채용Ficoll밀도제도리심법분리성인신선외주혈단핵세포,용세포배양기배양,연후분별용섬유련접단백、종류배사인자α유도24 h.용RT-PCR화면역세포화학방법검측단핵세포대림파관내피특이성표지물LYVE-1、Podoplanin、Prox1、혈관내피생장인자수체3이급내피세포항원vWF、내피형일양화담합매화혈관내피생장인자수체2적모인화단백표체.결과여결론:단핵세포재유도지전,대LYVE-1표체양성,대Podoplanin、Prox1、혈관내피생장인자수체3、vWF、내피형일양화담합매화혈관내피생장인자수체2표체균위음성;경상술인자자격후,단핵세포대Podoplanin、Prox-1、혈관내피생장인자수체3표체양성,vWF、내피형일양화담합매화혈관내피생장인자수체2표체잉정음성.제시섬유련접단백、종류배사인자α균능유효자격단핵세포표체림파관내피표지물.
BACKGROUND:Previous studies have shown that monocytes can transdifferentiate into vascular endothelial cells under the induction of various factors including vascular endothelial growth factor(VEGF).It remains poorly understood whether monocytes can be induced to transdifferentiate into lymphatic endothelial cells in vitro.OBJECTIVE:To explore the possibility of the transdifferentiation of monocytes into lymphatic endothelial cells under inflammatory condition.METHODS:Fresh monocytes from peripheral blood were collected by Ficoll density gradient centrifugation and cultured in an endothelial cell medium,followed by incubation in fibronectin-plated well or treated with tumor necrosis factor a for 24 hours,respectively.The expression of specific markers of lymphatic endothelial cells,such as LYVE-1,Podoplanin,Porx-1 and VEGF receptor 3(VEGFR-3),as well as the endothelial cells markers,such as vWF,endothelial nitric oxide synthase(eNOS)and VEGFR-2,were detected by RT-PCR and immunochemical methods.RESULTS AND CONCLUSION:Prior to induction,monocytes were positive to LYVE-1,but negative for Podoplanin,Porx-1,and VEGFR-3,vWF,eNOS,as well as VEGFR-2.Following induction,the cultured mononcytes were positive for Podoplanin,Prox-1 and VEGFR-3,but remained negative for vWF,eNOS and VEGFR-2.It suggested that monocytes can be induced to express the markers of lymphatic endothelial cells stimulated by fibronectin or tumor necrosis factor a.