CAJ | 학술논문

TasA是枯草芽孢杆菌(Bacillus subtilis)在芽孢的形成过程中产生的蛋白质,对多种动、植物病原菌有较强的抑制作用.从桑树内生枯草芽孢杆菌菌株ME0717基因组DNA中克隆到TasA基因的全序列,包含一个786 bp的完整开放阅读框(ORF), GenBank登录号为FJ713582.该序列与来源于B. subtilis的已知同源TasA序列Z99116、AJ871386的相似性达99.0%和98.1%,与芽孢形成相关的基因YqhF(GenBank登录号:BACJH642)的序列相似性也达99.0%,而与来源于地衣芽孢杆菌B. licheniformis的TasA序列(GenBank登录号:AE017333)相似性仅有46.0%,与来源于其它芽孢杆菌属的金属蛋白酶基因和芽孢外壳相关蛋白基因也有较高的相似性.构建该基因的原核表达载体,并在大肠杆菌(Escherichia coli)BL21中获得表达,表达产物对桑疫病病原细菌的菌体生长以及桑漆斑病菌和桑炭疽病菌的菌丝生长均有一定程度的抑制作用.
TasA시고초아포간균(Bacillus subtilis)재아포적형성과정중산생적단백질,대다충동、식물병원균유교강적억제작용.종상수내생고초아포간균균주ME0717기인조DNA중극륭도TasA기인적전서렬,포함일개786 bp적완정개방열독광(ORF), GenBank등록호위FJ713582.해서렬여래원우B. subtilis적이지동원TasA서렬Z99116、AJ871386적상사성체99.0%화98.1%,여아포형성상관적기인YqhF(GenBank등록호:BACJH642)적서렬상사성야체99.0%,이여래원우지의아포간균B. licheniformis적TasA서렬(GenBank등록호:AE017333)상사성부유46.0%,여래원우기타아포간균속적금속단백매기인화아포외각상관단백기인야유교고적상사성.구건해기인적원핵표체재체,병재대장간균(Escherichia coli)BL21중획득표체,표체산물대상역병병원세균적균체생장이급상칠반병균화상탄저병균적균사생장균유일정정도적억제작용.
TasA iS a protein produced during sporulation of Bacillus subtilis.It has strong inhibitory effect to many animal and plant pathogens.The full-length sequence of TasA gene was cloned from the genomic DNA of mulberry endophytic bacterium Bacillus subtilis strain ME071 7(GlenBank accession number FJ713582).The full length DNA sequence in-cludes a complete ORF of 786 base pairs.Compared with other TasA sequences previously reported,the gene had a high sequence similarity with those of B.subtilis.which was 99.0%with Z99116 and 98.1%with AJ871 386,while the similarity with the sporulation-associated gene YqhF(GenBank accession number BACJH642)reached 99.0%as well.Sequence similarity with TasA gene of B,licheniformis(GenBank accession number AE017333)was only 46.0%.The gene also has a high Ievel similarity with the genes of cell envelope-bound metalloprotease and spore coat-associated protein from Bacillus.The prokaryotic expression vector carrying TasA gene was constructed and expressed in Esche-richia coli BL21.The expressed protein showed certain inhibitory activity on growth of bacteriaI cells of Pseudomonas sy-ringae pv.mori and of mycelia of CoIletotrichum morifolium and Myrothecium roridum.