中国奶牛
中國奶牛
중국내우
CHINA DAIRY CATTLE
2011年
22期
55-58
,共4页
任倩然%张宇光%姜鹭%金君华%任发政
任倩然%張宇光%薑鷺%金君華%任髮政
임천연%장우광%강로%금군화%임발정
短芽孢杆菌%短杆菌素%溶氧%优化%分段培养
短芽孢桿菌%短桿菌素%溶氧%優化%分段培養
단아포간균%단간균소%용양%우화%분단배양
Bacillus Brevis%Tyrothricin%Dissolved oxygen%Optimization%Two stages with different oxygen supplement
本研究通过优化溶氧条件，对短芽孢杆菌（BacillusBrevis）（ATCC8185）进行分段培养，提高发酵液中短杆菌素产量。以析因试验确定菌体生成阶段培养条件为装液量35mL／100mL，摇床转速120r／min；以二次饱和D-最优设计试验确定短杆菌素合成阶段培养条件为：培养36h后静置培养。通过对溶氧条件的优化，发酵液中短杆菌素的产量达到616．93＋9．72μg／mL，比优化前提高了89％。
本研究通過優化溶氧條件，對短芽孢桿菌（BacillusBrevis）（ATCC8185）進行分段培養，提高髮酵液中短桿菌素產量。以析因試驗確定菌體生成階段培養條件為裝液量35mL／100mL，搖床轉速120r／min；以二次飽和D-最優設計試驗確定短桿菌素閤成階段培養條件為：培養36h後靜置培養。通過對溶氧條件的優化，髮酵液中短桿菌素的產量達到616．93＋9．72μg／mL，比優化前提高瞭89％。
본연구통과우화용양조건，대단아포간균（BacillusBrevis）（ATCC8185）진행분단배양，제고발효액중단간균소산량。이석인시험학정균체생성계단배양조건위장액량35mL／100mL，요상전속120r／min；이이차포화D-최우설계시험학정단간균소합성계단배양조건위：배양36h후정치배양。통과대용양조건적우화，발효액중단간균소적산량체도616．93＋9．72μg／mL，비우화전제고료89％。
This study analyzed the influence of dissolved oxygen on the yield of tyrothricin in flask culture. In order to improve the production of tyrothricin, two stages with different oxygen supplement were applied in the fermentation process of Bacillus Brevis （ATCC 8185）. The culture condition of growth stage were determined by factorial experiment as follows： liquid volume 35mL/ 100mL, rotate speed 120r/min. Through Second Saturating D-Optimal Design, the condition for synthesis stage was set as adjusting to stationary state at 36h of fermentation.After the optimization of dissolve oxygen in flask fermentation, the yield of tyrothricin was 616.93±9.72μg/mL and increased by 89% compared with previous one.
