中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2011年
6期
485-488
,共4页
谢芳%史学锋%许丽敏%张腾月%王嘉星%宁玉贤%赵堪兴
謝芳%史學鋒%許麗敏%張騰月%王嘉星%寧玉賢%趙堪興
사방%사학봉%허려민%장등월%왕가성%저옥현%조감흥
初级视皮层%双眼整合%场电位%猫
初級視皮層%雙眼整閤%場電位%貓
초급시피층%쌍안정합%장전위%묘
Primary cortex%Binocular integration%Local field potential%Kitten
背景 来自双眼视觉信号经视网膜、外侧膝状体直至初级视皮层融汇到单个细胞,构成双眼驱动细胞.双眼驱动细胞对双眼视觉信息进行整合和编码并介导高级视皮层产生融合和立体视觉,视觉发育关键期内的异常视觉经验常导致弱视和双眼整合反应的损害.目的 观察幼猫初级视皮层双眼驱动细胞对来自双眼视觉信号的整合反应特性.方法 选用3只8~10周健康幼猫,采用在体细胞外记录技术和双眼分视刺激方法,观察麻醉和肌肉麻痹状态下幼猫初级视皮层V1区28个电极记录部位的双眼驱动细胞对光栅刺激的峰电位发放率和局部场电位Gamma频率(20~90Hz)的振荡幅度,计算并比较两种信号的眼优势指数(ODI)和双眼整合指数(BII),观察细胞对双眼分别刺激的反应是否平衡及对双眼整合反应大小的影响.结果 在具双眼特性的28个细胞中,锋电位信号的眼优势绝对值较场电位的眼优势绝对值大(t=2.606,P=0.021);锋电位和场电位的ODI呈正相关(R2=0.513,F=27.423,P=0.003),双眼整合作用主要表现为易化,锋电位的BII(2.348±0.996)较场电位的BII(3.678±1.974)小,差异有统计学意义(t=2.671,P=0.019),对于锋电位信号,双眼平衡组的易化反应较双眼不平衡组高,差异有统计学意义(t=-2.519,P=0.035),对于场电位信号,双眼平衡组的易化反应较双眼不平衡组高,但差异无统计学意义(t=-1.671,P=0.146).结论 在正常发育的幼猫初级视皮层,神经元对来自双眼感受野内相同视刺激的整合作用主要表现为易化,且易化水平依赖于神经元对双眼分别的视刺激反应是否平衡,场电位信号和锋电位信号从不同皮层空间范围和来源成分反映了双眼整合的神经机制.
揹景 來自雙眼視覺信號經視網膜、外側膝狀體直至初級視皮層融彙到單箇細胞,構成雙眼驅動細胞.雙眼驅動細胞對雙眼視覺信息進行整閤和編碼併介導高級視皮層產生融閤和立體視覺,視覺髮育關鍵期內的異常視覺經驗常導緻弱視和雙眼整閤反應的損害.目的 觀察幼貓初級視皮層雙眼驅動細胞對來自雙眼視覺信號的整閤反應特性.方法 選用3隻8~10週健康幼貓,採用在體細胞外記錄技術和雙眼分視刺激方法,觀察痳醉和肌肉痳痺狀態下幼貓初級視皮層V1區28箇電極記錄部位的雙眼驅動細胞對光柵刺激的峰電位髮放率和跼部場電位Gamma頻率(20~90Hz)的振盪幅度,計算併比較兩種信號的眼優勢指數(ODI)和雙眼整閤指數(BII),觀察細胞對雙眼分彆刺激的反應是否平衡及對雙眼整閤反應大小的影響.結果 在具雙眼特性的28箇細胞中,鋒電位信號的眼優勢絕對值較場電位的眼優勢絕對值大(t=2.606,P=0.021);鋒電位和場電位的ODI呈正相關(R2=0.513,F=27.423,P=0.003),雙眼整閤作用主要錶現為易化,鋒電位的BII(2.348±0.996)較場電位的BII(3.678±1.974)小,差異有統計學意義(t=2.671,P=0.019),對于鋒電位信號,雙眼平衡組的易化反應較雙眼不平衡組高,差異有統計學意義(t=-2.519,P=0.035),對于場電位信號,雙眼平衡組的易化反應較雙眼不平衡組高,但差異無統計學意義(t=-1.671,P=0.146).結論 在正常髮育的幼貓初級視皮層,神經元對來自雙眼感受野內相同視刺激的整閤作用主要錶現為易化,且易化水平依賴于神經元對雙眼分彆的視刺激反應是否平衡,場電位信號和鋒電位信號從不同皮層空間範圍和來源成分反映瞭雙眼整閤的神經機製.
배경 래자쌍안시각신호경시망막、외측슬상체직지초급시피층융회도단개세포,구성쌍안구동세포.쌍안구동세포대쌍안시각신식진행정합화편마병개도고급시피층산생융합화입체시각,시각발육관건기내적이상시각경험상도치약시화쌍안정합반응적손해.목적 관찰유묘초급시피층쌍안구동세포대래자쌍안시각신호적정합반응특성.방법 선용3지8~10주건강유묘,채용재체세포외기록기술화쌍안분시자격방법,관찰마취화기육마비상태하유묘초급시피층V1구28개전겁기록부위적쌍안구동세포대광책자격적봉전위발방솔화국부장전위Gamma빈솔(20~90Hz)적진탕폭도,계산병비교량충신호적안우세지수(ODI)화쌍안정합지수(BII),관찰세포대쌍안분별자격적반응시부평형급대쌍안정합반응대소적영향.결과 재구쌍안특성적28개세포중,봉전위신호적안우세절대치교장전위적안우세절대치대(t=2.606,P=0.021);봉전위화장전위적ODI정정상관(R2=0.513,F=27.423,P=0.003),쌍안정합작용주요표현위역화,봉전위적BII(2.348±0.996)교장전위적BII(3.678±1.974)소,차이유통계학의의(t=2.671,P=0.019),대우봉전위신호,쌍안평형조적역화반응교쌍안불평형조고,차이유통계학의의(t=-2.519,P=0.035),대우장전위신호,쌍안평형조적역화반응교쌍안불평형조고,단차이무통계학의의(t=-1.671,P=0.146).결론 재정상발육적유묘초급시피층,신경원대래자쌍안감수야내상동시자격적정합작용주요표현위역화,차역화수평의뢰우신경원대쌍안분별적시자격반응시부평형,장전위신호화봉전위신호종불동피층공간범위화래원성분반영료쌍안정합적신경궤제.
Background The integration of segregated pathways from the two eyes first appears in V1 neurons,where it not only plays a critical role in the generation of a three-dimensional visual representation.Abnormal visual experiences in critical period usually lead to amblyopia and binocular integration defects.Objective Present study was to investigate how neurons of kitten coordinate their activity patterns in response to synchronous dichoptic stimulus inputs in striate cortex.Methods Spike rate and local field potential(LFP) gamma band(20-90Hz) power of three kitten(1-1.2Kg,8-10 weeks old) to monocular and synchronous dichoptic presented gratings were assessed for 28 binocular neurons in V1 of kitten by in vivo extracellular record method under anaesthesia and paralysis.Ocular dominance index(ODI) and binocular integration index(BII) were assessed and the correlation between these two indexes were analyzed.Results In 28 cells with binocular characteristic,the absolute value of spike-ODI was significant larger than that of LFP-ODI(t=2.606,P=0.021).A positive linear correlation between the ocular preferences of spike and LFP was found(R2=0.513,F=27.423,P=0.003).In dichotic trails,binocular facilitation with BII for spike was 2.348±0.996,showing a significant reduce in comparison with BII for LFP(3.678±1.974)(t=2.671,P=0.019).Binocular integration index for two signals were greater when monocular responses of both eyes were similar(P=0.035 and P=0.124,respectively).Conclusion Both spike rate and gamma band power of LFP exhibited binocular facilitation to synchronous presented dichotic stimuli with significant facilitation induced by balanced monocular responses.Spiking activity and LFP reflect neural activities of different spatial scales and source components.