中国小儿急救医学
中國小兒急救醫學
중국소인급구의학
CHINESE PEDIATRIC EMERGENCY MEDICINE
2010年
3期
242-243,247
,共3页
血管内皮生长因子%脑膜炎%脑脊液%地塞米松%家兔
血管內皮生長因子%腦膜炎%腦脊液%地塞米鬆%傢兔
혈관내피생장인자%뇌막염%뇌척액%지새미송%가토
Vascular endothelial growth factor%Meningitis%Cerebrospinal fluid%Dexamethasone%Rabbit
目的 探讨地塞米松(DEX)对脑膜炎家兔脑脊液(CSF)中血管内皮生长因子(VEGF)质量浓度变化的影响,为临床应用DEX辅助治疗脑膜炎提供理论依据.方法 家兔36只,随机分为模型组、DEX处理组和对照组.于注射大肠埃希菌悬液后6、12及24 h采用双抗夹心ELISA法定量检测兔CSF中VEGF的质量浓度.结果 模型组6、12及24h CSF中VEGF质量浓度[(1 219±176)ng/L、(1 343±160)ng/L、(981±134)ng/L]明显高于同时相对照组[(374±172)ng/L、(370±169)ng/L、(367±171)ng/L],差异有非常显著性(P<0.01);脑组织含水量高于对照组[(80.8±0.5)%vs(80.0±0.5)%],差异有非常显著性(P<0.01);脑组织含水量与24 h CSF中VEGF质量浓度呈正相关(r=0.919,P<0.01).DEX处理组6、12及24 h CSF中VEGF质量浓度[(941±147)ng/L、(1 083±123)ng/L、(825±66)ng/L]低于同时相模型组,差异有显著性(P<0.05),脑组织含水量(80.4±0.5)%低于模型组,差异有显著性(P<0.05).结论 细菌性脑膜炎病程中VEGF分泌增多,VEGF参与脑水肿形成;DEX通过抑制VEGF表达和(或)分泌,减轻血脑屏障损害,抑制脑水肿形成.
目的 探討地塞米鬆(DEX)對腦膜炎傢兔腦脊液(CSF)中血管內皮生長因子(VEGF)質量濃度變化的影響,為臨床應用DEX輔助治療腦膜炎提供理論依據.方法 傢兔36隻,隨機分為模型組、DEX處理組和對照組.于註射大腸埃希菌懸液後6、12及24 h採用雙抗夾心ELISA法定量檢測兔CSF中VEGF的質量濃度.結果 模型組6、12及24h CSF中VEGF質量濃度[(1 219±176)ng/L、(1 343±160)ng/L、(981±134)ng/L]明顯高于同時相對照組[(374±172)ng/L、(370±169)ng/L、(367±171)ng/L],差異有非常顯著性(P<0.01);腦組織含水量高于對照組[(80.8±0.5)%vs(80.0±0.5)%],差異有非常顯著性(P<0.01);腦組織含水量與24 h CSF中VEGF質量濃度呈正相關(r=0.919,P<0.01).DEX處理組6、12及24 h CSF中VEGF質量濃度[(941±147)ng/L、(1 083±123)ng/L、(825±66)ng/L]低于同時相模型組,差異有顯著性(P<0.05),腦組織含水量(80.4±0.5)%低于模型組,差異有顯著性(P<0.05).結論 細菌性腦膜炎病程中VEGF分泌增多,VEGF參與腦水腫形成;DEX通過抑製VEGF錶達和(或)分泌,減輕血腦屏障損害,抑製腦水腫形成.
목적 탐토지새미송(DEX)대뇌막염가토뇌척액(CSF)중혈관내피생장인자(VEGF)질량농도변화적영향,위림상응용DEX보조치료뇌막염제공이론의거.방법 가토36지,수궤분위모형조、DEX처리조화대조조.우주사대장애희균현액후6、12급24 h채용쌍항협심ELISA법정량검측토CSF중VEGF적질량농도.결과 모형조6、12급24h CSF중VEGF질량농도[(1 219±176)ng/L、(1 343±160)ng/L、(981±134)ng/L]명현고우동시상대조조[(374±172)ng/L、(370±169)ng/L、(367±171)ng/L],차이유비상현저성(P<0.01);뇌조직함수량고우대조조[(80.8±0.5)%vs(80.0±0.5)%],차이유비상현저성(P<0.01);뇌조직함수량여24 h CSF중VEGF질량농도정정상관(r=0.919,P<0.01).DEX처리조6、12급24 h CSF중VEGF질량농도[(941±147)ng/L、(1 083±123)ng/L、(825±66)ng/L]저우동시상모형조,차이유현저성(P<0.05),뇌조직함수량(80.4±0.5)%저우모형조,차이유현저성(P<0.05).결론 세균성뇌막염병정중VEGF분비증다,VEGF삼여뇌수종형성;DEX통과억제VEGF표체화(혹)분비,감경혈뇌병장손해,억제뇌수종형성.
Objective To investigate the effects of dexamethasone (DEX) on vascular endothelial growth factor( VEGF) in cerebrospinal fluid of rabbits with bacterial meningitis. Methods A total of 36 rabbits were assigned to study,which were randomly divided into meningitis model group (MOD) .dexametha-sone-treated group (DEXT) and control group( CON). CSF was sampled for determining at 6 h, 12 h and 24 h after injection of E. coli suspension. The concentration of VEGF in every CSF sample was determined quantitatively by ELISA. Results There were higher concentrations of CSF VEGF at6h,12h and 24hin M0D(( 1219 ±176) ng/L,( 1343 ±160) ng/L,(981 ±134) ng/L) than that in CON( (374 ±172) ng/L, (370 ± 169) ng/L,(367 ± 171) ng/L) (P<0.01). There was higher brain water content in M0D( (80.8 ± 0.5) % ) than that in CON( (80.0 ± 0.5) % ) (P < 0.01). There was positive correlation between the brain water content and the concentration of CSF VEGF at 24 h( r - 0.919,P < 0.01). Compared with MOD, the concentrations of CSF VEGF in DEXT at 6 h, 12 h,24 h ((941 ±147) ng/L, (1083 ± 123) ng/L, (825 ± 66) ng/L) were decreased significantly(P <0.05), the brain water content was less ((80.4 ±0.5) %) (P < 0.05). Conclusion The secretion of VEGF markedly increases in the pathological process of bacterial meningitis. VEGF contributes to the damage of blood brain barrier and the formation of brain edema. DEX can decrease the degree of brain edema by suppressing the generation of VEGF and lightening the damage of blood brain barrier.
