CAJ | 학술논문

目的探讨姜黄素对大鼠神经病理性痛的影响.方法健康雄性SD大鼠108只,体重220～250 g,随机分为6组(n=18):对照组(C组)、假手术组(S组)、慢性压迫性损伤组(CCI组)和不同剂量姜黄素组(Cur1组、Cur2组和Cur3组).C组腹腔注射二甲基亚砜4 ml·kg-1·d-1,持续14 d;S组只分离坐骨神经,然后腹腔注射二甲基亚砜4 ml·kg-1·d-1,持续至术后14 d;CCI组术后腹腔注射二甲基亚砜4 ml·kg-1·d-1,持续至术后14 d;Cur1组、Cur2组或Cur3组术后分别腹腔注射姜黄素30、100、300 mg·kg-1·d-1,持续至术后14 d,姜黄素以二甲基亚砜溶解.于术前2 d和术后1、3、5、7、10、14d时测定热缩足反应潜伏期(TWL)和机械缩足反应阚值(MWT).各组于术后3、7、14 d时各处死6只大鼠,免疫组化法测定脊髓背角和背根神经节中磷酸化c-jun氨基末端蛋白激酶(p-JNK)和c-jun的表达.结果与CCI组比较,Cur1组和Cur2组术后MWT升高,背根神经节和脊髓背角p-JNK和c-jun表达下调,Cur2组术后TWL升高(P<0.05);与Cur1组比较,Cur2组术后TWL升高,背根神经节和脊髓背角p-JNK和c-jun表达均下调(P<0.05),MWT差异无统计学意义(P0.05).结论姜黄素30、100mg/kg可减轻大鼠神经病理性痛,100 mg/kg效果更明显,其机制可能与抑制脊髓背角和背根神经节p-JNK和c-jun表达上调有关.
목적 탐토강황소대대서신경병이성통적영향.방법 건강웅성SD대서108지,체중220～250 g,수궤분위6조(n=18):대조조(C조)、가수술조(S조)、만성압박성손상조(CCI조)화불동제량강황소조(Cur1조、Cur2조화Cur3조).C조복강주사이갑기아풍4 ml·kg-1·d-1,지속14 d;S조지분리좌골신경,연후복강주사이갑기아풍4 ml·kg-1·d-1,지속지술후14 d;CCI조술후복강주사이갑기아풍4 ml·kg-1·d-1,지속지술후14 d;Cur1조、Cur2조혹Cur3조술후분별복강주사강황소30、100、300 mg·kg-1·d-1,지속지술후14 d,강황소이이갑기아풍용해.우술전2 d화술후1、3、5、7、10、14d시측정열축족반응잠복기(TWL)화궤계축족반응감치(MWT).각조우술후3、7、14 d시각처사6지대서,면역조화법측정척수배각화배근신경절중린산화c-jun안기말단단백격매(p-JNK)화c-jun적표체.결과 여CCI조비교,Cur1조화Cur2조술후MWT승고,배근신경절화척수배각p-JNK화c-jun표체하조,Cur2조술후TWL승고(P<0.05);여Cur1조비교,Cur2조술후TWL승고,배근신경절화척수배각p-JNK화c-jun표체균하조(P<0.05),MWT차이무통계학의의(P0.05).결론 강황소30、100mg/kg가감경대서신경병이성통,100 mg/kg효과경명현,기궤제가능여억제척수배각화배근신경절p-JNK화c-jun표체상조유관.
Objective To investigate the effect of curcumin on neurepathic pain in rats. Methods One hundred and eight male SD rats weighing 220-250 g were randomly divided into 6 groups (n=18 each): Ⅰ control group (C);Ⅱ sham operation group (S);Ⅲ chronic constrictive injury group (CCI) and Ⅳ,Ⅴ,Ⅵ 3 curcumin treatment groups (Cur<1-3). CCI was produced by placing 3-4 ligatures with 4-0 chromic catgut on right sciatic nerve at 1 mm intervals. Curcumin 30, 100 and 300 mg/kg were given intraperitoneally (IP) every day for 14 days after operation in group Cur1-3. Curcumin was dissolved in DMSO, of which 4 ml/kg was given IP every day for 14 days in group C, S and CCI. Thermal paw-withdrawal latency (TWL) and mechanical paw-withdrawal threshold (MWT) were measured at day 2 before operation and day 1, 3, 5, 7, 10 and 14 after operation. The animals were deeply anesthetized and decapitated at day 3, 7, 14 after operation. The lumbar segment (L4,5) of the spinal cord and dorsal root ganglions (DRGs) were removed for determination of p-JNK and c-jun expression by immuno-histochemistry. Results Curcumin of 30,100 mg/kg significantly increased the MWT and TWL as compared with CCI group. The expression of p-JNK and c-jun in the spinal cord and DRGs was significantly increased in CCI group. Curcumin of 30,100 mg/kg significantly decreased the expression of p-JNK and e-jun in the spinal cord and DRGs as compared with CCI group. Conclusion Curcumin of 30,100 mg/kg can relieve neurepathic pain by inhibiting the up-regulation of p-JNK and c-jun expression in the spinal cord and DRGs.