CAJ | 학술논문

目的研究低压缺氧、+Gz暴露等飞行环境因素对肾结石形成的影响. 方法 48只Wistar雄性大鼠用随机区组法分为对照组、低压缺氧组、+Gz暴露组和结石模型组,每组12只.施加相应干预因素饲养4周以后,检验各组大鼠血清生化指标:血尿素氮、血肌酐、血磷和血钙;尿液相关指标:24小时尿量、尿pH值、尿钙、尿酸和草酸含量.镜下观察大鼠肾苏木精和伊红( hematoxylin-eosin,HE)染色组织切片中结石结晶及肾小管扩张情况. 结果 ①+Gz暴露组及结石模型组大鼠血清尿素氮含量高于对照组(P＜0.05),其它指标组间差异无统计学意义.②各组大鼠24 h尿量和尿钙含量差异无统计学意义；大鼠尿液尿酸含量组间差异有统计学意义(F=33.064,P＜0.01),+Gz暴露组及结石模型组明显高于对照组(P＜0.01)；尿草酸含量组间差异有统计学意义(F=4.877,P＜0.01),+Gz暴露组及结石模型组明显高于对照组(P＜0.05);各组大鼠尿pH值差异有统计学意义(F=3.555,P＜0.05),低压缺氧组大鼠尿pH值比对照组降低(P＜0.05).③对照组未见肾小管扩张及结晶体沉积；低压缺氧组未见肾小管扩张,其内可见极少量结石结晶；+Gz暴露组肾小管扩张不明显,其内可见无色透明的结石结晶；结石模型组肾小管扩张增粗,其内可见大量无色透明的结石结晶呈片状分布. 结论 +Gz暴露可能是泌尿系结石形成的危险因素之一,而低压缺氧对泌尿系结石形成的影响不明显.
목적 연구저압결양、+Gz폭로등비행배경인소대신결석형성적영향. 방법 48지Wistar웅성대서용수궤구조법분위대조조、저압결양조、+Gz폭로조화결석모형조,매조12지.시가상응간예인소사양4주이후,검험각조대서혈청생화지표:혈뇨소담、혈기항、혈린화혈개;뇨액상관지표:24소시뇨량、뇨pH치、뇨개、뇨산화초산함량.경하관찰대서신소목정화이홍( hematoxylin-eosin,HE)염색조직절편중결석결정급신소관확장정황. 결과 ①+Gz폭로조급결석모형조대서혈청뇨소담함량고우대조조(P＜0.05),기타지표조간차이무통계학의의.②각조대서24 h뇨량화뇨개함량차이무통계학의의；대서뇨액뇨산함량조간차이유통계학의의(F=33.064,P＜0.01),+Gz폭로조급결석모형조명현고우대조조(P＜0.01)；뇨초산함량조간차이유통계학의의(F=4.877,P＜0.01),+Gz폭로조급결석모형조명현고우대조조(P＜0.05);각조대서뇨pH치차이유통계학의의(F=3.555,P＜0.05),저압결양조대서뇨pH치비대조조강저(P＜0.05).③대조조미견신소관확장급결정체침적；저압결양조미견신소관확장,기내가견겁소량결석결정；+Gz폭로조신소관확장불명현,기내가견무색투명적결석결정；결석모형조신소관확장증조,기내가견대량무색투명적결석결정정편상분포. 결론 +Gz폭로가능시비뇨계결석형성적위험인소지일,이저압결양대비뇨계결석형성적영향불명현.
Objective To investigate the effects of flight environmental factors,such as hypobaric hypoxia or +Gz exposure on the formation of kidney stone. Methods Forty-eight adult male Wistar rats were randomly and averagely assigned into control,hypobaric hypoxia, + Gz exposure and stone formation group.The rats of hypobaric hypoxia group were tested in 5000 m equivalent hypoxia environment for 1 h per 3 times a week for 4 weeks.+ Gz exposure group was exposed in +9 Gz for 25 s,5 times/d (with 5 min interval) by centrifuge.The frequency of +Gz exposure was 3 times a week for 4 weeks.The stone formation group was fed with water with 1％ ethylene glycol and poured with 2％ ammonium chloride by the dose of 2 ml/d for each.The serum blood urea nitrogen (BUN),creatinine (Scr),phosphorus(Pi),Ca2+,24 h urine volume,urinary excretion of Ca2+,oxalare (Ox),uric acid and urine pH value were detected.All rats' kidneys were removed and stained with hematoxylin-eosin (HE),and then examined by microscope to check the possible calculi crystals and tubular dilatation. Results ① The concentrations of BUN in + Gz exposure group and stone formation group were higher than that of control group (P＜0.05).Other data showed no difference among all groups. ② 24 h urine volume and Ca2+ of urine were no significant difference among groups but the uric acid (F=33.064,P＜0.01).Uric acid of urine of +Gz exposure group and stone formation group was significantly higher than that of control group (P＜0.01).Significant difference on oxalate of urine was observed among all groups (F=4.877,P＜0.01).Oxalate of urine of +Gz exposure group and stone formation group was higher than that of control group (P＜0.05).Urine pH value of hypobaric hypoxia group was lower than that of control group (t=3.099,P＜0.05).③ Renal tubules were normal and no calculi crystal was found in control group.Renal tubules were normal and only slight amount of calculi crystals were found in hypobaric hypoxia group.In +Gz exposure group,transparent scattered crystals could be seen and the tubules were slightly dilated.Big amount of transparent crystals could be seen in lumen of the evidently dilated tubules in stone formation group. Conclusions Acceleration would be one of the risk factors contributed to kidney stone formation.But the effect of hypobaric hypoxia on the formation of kidney stone is not obvious.