CAJ | 학술논문

目的探讨补肾益气药预防家兔膝骨性关节炎的机制.方法将72只4月龄健康日本长耳大白兔,随机分为空白组(A组)、模型组(B组)、中药高剂量组(C组)、中药中剂量组(D组)、中药小剂量组(E组)、盐酸氨基葡萄糖胶囊对照组(F组)6组各12只.除A组外,均采用石膏管形固定膝关节6周,制作兔膝骨性关节炎模型.在造模同时C、D、E、F组分别给予不同剂量中药、盐酸氨基葡萄糖胶囊灌胃4周.模型组给予用药组等容的生理盐水灌胃;空白组不灌胃.造模6周后抽取关节液,应用ELISA法测定关节液中肿瘤坏死因子(TNF-α)的变化.同时取关节软骨行大体及光镜检查.结果组织学显示,C、D、E、F组膝关节软骨有不同程度的色泽变化,骨赘、骨囊肿形成,软骨关节面出现浅表性糜烂,部分标本软骨缺损深达软骨中层,出现软骨剥脱,但程度轻于B组.与A组比较,B组关节液中TNF-α含量明显升高(P＜0.05),表明造模成功;C、D、E、F组的TNF-α含量均低于B组,差异有统计学意义(P均＜0.05).C、D、E不同剂量组间比较,差异有统计学意义,即随用药剂量增大,兔关节液中TNF-α含量越低.结论补肾益气药可能通过降低兔关节液中TNF-α含量预防骨性关节炎.
목적 탐토보신익기약예방가토슬골성관절염적궤제.방법 장72지4월령건강일본장이대백토,수궤분위공백조(A조)、모형조(B조)、중약고제량조(C조)、중약중제량조(D조)、중약소제량조(E조)、염산안기포도당효낭대조조(F조)6조각12지.제A조외,균채용석고관형고정슬관절6주,제작토슬골성관절염모형.재조모동시C、D、E、F조분별급여불동제량중약、염산안기포도당효낭관위4주.모형조급여용약조등용적생리염수관위;공백조불관위.조모6주후추취관절액,응용ELISA법측정관절액중종류배사인자(TNF-α)적변화.동시취관절연골행대체급광경검사.결과 조직학현시,C、D、E、F조슬관절연골유불동정도적색택변화,골췌、골낭종형성,연골관절면출현천표성미란,부분표본연골결손심체연골중층,출현연골박탈,단정도경우B조.여A조비교,B조관절액중TNF-α함량명현승고(P＜0.05),표명조모성공;C、D、E、F조적TNF-α함량균저우B조,차이유통계학의의(P균＜0.05).C、D、E불동제량조간비교,차이유통계학의의,즉수용약제량증대,토관절액중TNF-α함량월저.결론 보신익기약가능통과강저토관절액중TNF-α함량예방골성관절염.
Objective To explore the mechanism of preventive effect of Chinese medicine with Replenishing kidney and invigorating Qi on osteoarthritis. Methods 72 male Long-eared white rabbits aged 4 months were randomly divided into six groups, A group (blank control group), B group (model group), C group (traditional Chinese medicine high-dose prevention group), D group (Chinese herbal medicine with medium-dose prevention group), E group (Chinese herbal medicine with low-dose prevention group), F group (glucose-amino acid hydrochloride capsules prevention group).All the animals apart from A group were established osteoarthritis model by immobilizing knee joint with plaster cast for 6 weeks. In the same time of immobilization, traditional Chinese medicine and glucose-amino acid hydrochloride capsules were given to C, D, E and F group for 4 weeks. Physiological saline was given to B group. After 6 weeks of modeling, synovial fluid was extracted and the changes of TNF-α in it were analyzed by ELISA. Results Articular cartilage degeneration of B group was most obviously compared with C, D, E and F group. There was a significant difference of TNF-α level in comparison of A and B group (P＜0.05), B and C, D, E and F group (P＜0.05), and among C, D and E group. Conclusion Chinese medicine with replenishing kidney and invigorating Qi can prevent osteoarthritis by reducing TNF-α level in synovial fluid, enhancing cartilage cell metabolism, and slowing down cartilage degradation.