CAJ | 학술논문

目的探讨缺氧诱导因子-1α(HIF-1α)在卵巢子宫内膜异位症组织中表达的意义.方法应用Elivision Tm plus免疫组化方法分别检测30例卵巢子宫内膜异位症异位、在位内膜和非子宫内膜异位症(Ems)子宫内膜组织(对照组)中的HIF-1α,并应用计算机图像分析系统对结果进行体视学指标平均吸光度的检测.结果卵巢子宫内膜异位症组异位内膜、在位内膜及对照组中HIF-1α的平均吸光度分别为(0.077±0.014)、(0.070±0.013)及(0.061±0.007),3组比较差异有统计学意义(F=37.738,P＜0.01＝.HIF-1α蛋白在卵巢子宫内膜异位症组在位内膜增生期的表达(0.076±0.007)高于分泌期(0.059±0.014)(t=6.918,P＜0.01＝,高于对照组同期水平(0.060±0.007)(t=12.724,P＜0.01＝.结论 HIF-1可能与卵巢子宫内膜异位症的发生、发展密切相关.
목적 탐토결양유도인자-1α(HIF-1α)재란소자궁내막이위증조직중표체적의의.방법 응용Elivision Tm plus면역조화방법분별검측30례란소자궁내막이위증이위、재위내막화비자궁내막이위증(Ems)자궁내막조직(대조조)중적HIF-1α,병응용계산궤도상분석계통대결과진행체시학지표평균흡광도적검측.결과 란소자궁내막이위증조이위내막、재위내막급대조조중HIF-1α적평균흡광도분별위(0.077±0.014)、(0.070±0.013)급(0.061±0.007),3조비교차이유통계학의의(F=37.738,P＜0.01＝.HIF-1α단백재란소자궁내막이위증조재위내막증생기적표체(0.076±0.007)고우분비기(0.059±0.014)(t=6.918,P＜0.01＝,고우대조조동기수평(0.060±0.007)(t=12.724,P＜0.01＝.결론 HIF-1가능여란소자궁내막이위증적발생、발전밀절상관.
Objective To investigate the significance of hypoxia inducible factor-1(HIF-1) expression in ovarian endometriosis (Oems). Methods Elivision Tm plus Immunohistochemical and Stereology methods were used to detect HIF-1 expression in 30 cases of ectopic endometrium, 30 cases of eutopic endometrium in ovarian endometriosis and 30 cases of endometrium in ovarian teratoma as the normal control. Quantitative images analysis was performed with the aid of computer to collect and analyze the mean optical density (MOD) of HIF-1 expression. Results The MOD values of HIF-1 in ectopic and eutopic endometrium of Oems and the normal control were 0. 077 ± 0. 014, 0. 070 ± 0. 013 and 0. 061 ± 0. 007 respectively, which showed significantly differences between each other groups(F = 37. 738, P ＜ 0. 01 ＝. HIF-1 expression in proliferative phase (0.076 ±0.007) of eutopic endometrium of Oems was significantly higher than that in secretary phase (0. 059 ±0. 014) (t = 6. 918 ,P ＜0. 01 ＝; which was also significantly higher than that of the normal control in the same phase (0.060±0.007) (t=-12.724,P ＜0.01 ＝.Conclusion HIF-1 may play an important role in the pathogenesis and development of Oems.