CAJ | 학술논문

目的探讨Toll样受体2、4 mRNA在麻风患者外周血单一核细胞中的表达.方法运用SYBR Green荧光定量RT-PCR技术检测麻风现症患者与已痊愈患者各30例外周血单一核细胞中Toll样受体2、4 mRNA的表达水平,并用正常人作对照.结果 Toll样受体2 mRNA在麻风患者外周血单一核细胞中表达明显高于痊愈组和正常人对照组(P＜0.01).Toll样受体4 mRNA在麻风现症患者外周血单一核细胞中的表达与痊愈组和正常人对照组差异无统计学意义(P＞0.05).结论 Toll样受体2 mRNA在麻风患者外周血单一核细胞的表达明显升高,提示Toll样受体2 mRNA可能是麻风杆菌的特异识别受体.
목적 탐토Toll양수체2、4 mRNA재마풍환자외주혈단일핵세포중적표체.방법 운용SYBR Green형광정량RT-PCR기술검측마풍현증환자여이전유환자각30예외주혈단일핵세포중Toll양수체2、4 mRNA적표체수평,병용정상인작대조.결과 Toll양수체2 mRNA재마풍환자외주혈단일핵세포중표체명현고우전유조화정상인대조조(P＜0.01).Toll양수체4 mRNA재마풍현증환자외주혈단일핵세포중적표체여전유조화정상인대조조차이무통계학의의(P＞0.05).결론 Toll양수체2 mRNA재마풍환자외주혈단일핵세포적표체명현승고,제시Toll양수체2 mRNA가능시마풍간균적특이식별수체.
Objective To study the mRNA expressions of Toll-like receptor (TLR) 2 and 4 in peripheral blood mononuclear cells (PBMCs) of patients with leprosy. Methods SYBR Green fluorescence quantitative RT-PCR was conducted to detect the mRNA expressions of TLR2 and 4 in PBMCs from 30 patients with cured leprosy, 30 patients with active leprosy and 30 normal human controls. Results The expression of TLR2 mRNA in patients with active leprosy was significantly higher than that in those with cured leprosy and in controls (both P ＜ 0.01 ), but there was no significant difference between the latter two groups (P ＞ 0.05). The expression of TLR4 mRNA was of no significant difference between patients with active leprosy and those with cured leprosy orthe controls (both P＞ 0.05). Conclusion The expression of TLR2 mRNA is decreased in PBMCs from leprosy patients, suggesting that TLR2 may be a specific recognition receptor in patients with leprosy.