中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2009年
7期
648-651
,共4页
姚俊岩%王泉云%翁浩%张兰%张艳丽
姚俊巖%王泉雲%翁浩%張蘭%張豔麗
요준암%왕천운%옹호%장란%장염려
二异丙酚%再灌注损伤%脊髓%细胞凋亡
二異丙酚%再灌註損傷%脊髓%細胞凋亡
이이병분%재관주손상%척수%세포조망
Propofol%Reperfnsion injury%Spired cord%Apotosis
目的 探讨异丙酚对兔脊髓缺血再灌注时脊髓前角神经细胞凋亡的影响.方法 新西兰大耳白兔60只,月龄4～6月,体重2.0～2.5 kg,随机分为6组(n=10):对照组(C组)、10%脂肪乳组(F组)、异丙酚30 mg/kg(P1)组、异丙酚40 mg/kg(P2)组、异丙酚50 mg/kg(P3)组和异丙酚60 mg/kg(P4)组.P1组、P2组、P3组和P4组异丙酚用10%脂肪乳稀释至6 ml/kg,C组和F组给予等容量生理盐水或脂肪乳.全麻下开腹阻断左肾动脉远端的腹主动脉及双侧髂总动脉30 min进行脊髓缺血,自缺血即刻开始,各组以12 ml·kg-1·h-1的速率经股动脉输注生理盐水(C组)、10%脂肪乳(F组)和不同剂量异丙酚(P1～4组),30 min后停止输注,开放腹主动脉行再灌注.再灌注48 h时,取L4～6脊髓组织,光镜下计数脊髓前角正常运动神经细胞;采用TUNEL法计数脊髓前角总细胞和凋亡细胞,计算细胞凋亡指数;采用免疫组化法测定脊髓前角cagpase-3表达.结果 与C组和F组比较,P1～4组脊髓前角正常运动神经元计数升高,凋亡指数降低,caspase-3表达下调(P<0.05);与P1组比较,P2～4组脊髓前角正常运动神经元计数升高,凋亡指数降低,P3组和P4组cagpase-3表达下调(P<0.05);与P2组比较,P3组脊髓前角正常运动神经元计数升高,P4组降低,P3组和P4组凋亡指数降低,caspase-3表达下调(P<0.05);与P3组比较,P4组脊髓前角正常运动神经元计数降低,凋亡指数升高,cagpage-3表达上调(P<0.05).结论 腹主动脉阻断期间,经腹主动脉输注30～60 mg/kg异丙酚可抑制脊髓前角神经细胞凋亡,从而减轻兔脊髓缺血再灌注损伤,且与剂量有关,其机制与下调脊髓cagpage-3表达有关.
目的 探討異丙酚對兔脊髓缺血再灌註時脊髓前角神經細胞凋亡的影響.方法 新西蘭大耳白兔60隻,月齡4～6月,體重2.0～2.5 kg,隨機分為6組(n=10):對照組(C組)、10%脂肪乳組(F組)、異丙酚30 mg/kg(P1)組、異丙酚40 mg/kg(P2)組、異丙酚50 mg/kg(P3)組和異丙酚60 mg/kg(P4)組.P1組、P2組、P3組和P4組異丙酚用10%脂肪乳稀釋至6 ml/kg,C組和F組給予等容量生理鹽水或脂肪乳.全痳下開腹阻斷左腎動脈遠耑的腹主動脈及雙側髂總動脈30 min進行脊髓缺血,自缺血即刻開始,各組以12 ml·kg-1·h-1的速率經股動脈輸註生理鹽水(C組)、10%脂肪乳(F組)和不同劑量異丙酚(P1～4組),30 min後停止輸註,開放腹主動脈行再灌註.再灌註48 h時,取L4～6脊髓組織,光鏡下計數脊髓前角正常運動神經細胞;採用TUNEL法計數脊髓前角總細胞和凋亡細胞,計算細胞凋亡指數;採用免疫組化法測定脊髓前角cagpase-3錶達.結果 與C組和F組比較,P1～4組脊髓前角正常運動神經元計數升高,凋亡指數降低,caspase-3錶達下調(P<0.05);與P1組比較,P2～4組脊髓前角正常運動神經元計數升高,凋亡指數降低,P3組和P4組cagpase-3錶達下調(P<0.05);與P2組比較,P3組脊髓前角正常運動神經元計數升高,P4組降低,P3組和P4組凋亡指數降低,caspase-3錶達下調(P<0.05);與P3組比較,P4組脊髓前角正常運動神經元計數降低,凋亡指數升高,cagpage-3錶達上調(P<0.05).結論 腹主動脈阻斷期間,經腹主動脈輸註30～60 mg/kg異丙酚可抑製脊髓前角神經細胞凋亡,從而減輕兔脊髓缺血再灌註損傷,且與劑量有關,其機製與下調脊髓cagpage-3錶達有關.
목적 탐토이병분대토척수결혈재관주시척수전각신경세포조망적영향.방법 신서란대이백토60지,월령4～6월,체중2.0～2.5 kg,수궤분위6조(n=10):대조조(C조)、10%지방유조(F조)、이병분30 mg/kg(P1)조、이병분40 mg/kg(P2)조、이병분50 mg/kg(P3)조화이병분60 mg/kg(P4)조.P1조、P2조、P3조화P4조이병분용10%지방유희석지6 ml/kg,C조화F조급여등용량생리염수혹지방유.전마하개복조단좌신동맥원단적복주동맥급쌍측가총동맥30 min진행척수결혈,자결혈즉각개시,각조이12 ml·kg-1·h-1적속솔경고동맥수주생리염수(C조)、10%지방유(F조)화불동제량이병분(P1～4조),30 min후정지수주,개방복주동맥행재관주.재관주48 h시,취L4～6척수조직,광경하계수척수전각정상운동신경세포;채용TUNEL법계수척수전각총세포화조망세포,계산세포조망지수;채용면역조화법측정척수전각cagpase-3표체.결과 여C조화F조비교,P1～4조척수전각정상운동신경원계수승고,조망지수강저,caspase-3표체하조(P<0.05);여P1조비교,P2～4조척수전각정상운동신경원계수승고,조망지수강저,P3조화P4조cagpase-3표체하조(P<0.05);여P2조비교,P3조척수전각정상운동신경원계수승고,P4조강저,P3조화P4조조망지수강저,caspase-3표체하조(P<0.05);여P3조비교,P4조척수전각정상운동신경원계수강저,조망지수승고,cagpage-3표체상조(P<0.05).결론 복주동맥조단기간,경복주동맥수주30～60 mg/kg이병분가억제척수전각신경세포조망,종이감경토척수결혈재관주손상,차여제량유관,기궤제여하조척수cagpage-3표체유관.
Objective To investigate the effects of propofol on neuronal apoptosis in anterior horn of spinal cord in rabbits with spinal cord ischemia-reperfusion (IR) injury. Methods Sixty New Zealand white rabbits aged 4-6 months weighing 2.0-2.5 kg were randomized to receive normal saline (group C), 10% intralipid (group F) and propofol 30 mg/kg (group P1 ), 40 mg/kg (group P2), 50 mg/kg (group P3) and60 mg/kg (group P4 ). 10% intralipid was added to propofol solution to make the fluid infused equal in volume between the 6 groups ( n = 10 each). Spinal cord ischemia was induced by occlusion of abdominal aorta distal to the left renal arteries combined with simultaneous occlusion of bilateral common iliac arteries for 30 min. A catheter was inserted into abdominal aorta close to the site of occlusion via left femoral artery. Normal saline, 10% intralipid or different doses of propofol was infused through the catheter as soon as aorta was clamped at the rate of 12 ml·kg-1·h-1 for 30 min. The aorta and bilateral iliac arteries were then declamped. The L4-6 of spinal cord was removed at 48 h of reperfusion for microscopic examination and the total number of normal motor neurons in the anterior horn of spinal cord was counted. The total number of neurons and apoptosis neurons in the anterior horn of spinal cord was counted by TUNEL and the apoptosis index of neurons was calculated. The expression of caspase-3 in the anterior horn of spinal cord was determined by immunohistochemical technique. Results The number of normal motor neurons was significantly higher, and the apoptosis index and expression of caspase-3 were significantly lower in group P1-4 than in group C and F ( P < 0.05). Compared with group P1, the number of normal motor neurons was significantly increased and the apoptosis index was significantly decreased in group P2-4 and the expression of caspase-3 was down-regulated in group P3 and P4 ( P < 0.05). Compared with group P2, the number of normal motor neurons was significantly increased in group P3 while decreased in group P4, and the apoptosis index was significantly decreased and the expression of caspase-3 was down-regulated in group P3 and P4 ( P < 0.05). Compared with group P3, the number of normal motor neurons was significantly decreased and the apoptosis index was significantly increased and the expression of easpnse-3 was up-regulated in group P4 ( P < 0.05) . Conclusion Propofol 30-60 mg/kg infused through aorta during occlusion can inhibit the neuronal apoptosis and attenuate IR injury to spinal cord dose-dependently in rabbits. The underlying mechanism may be related to the down-regulation of caspase-3 expression.
