CAJ | 학술논문

目的观察氯化锂联合移植自体激活雪旺细胞(AASCs)在治疗脊髓损伤(SCI)中的作用.方法 Wistar成鼠48只建立T10急性SCI模型.随机均分为4组:AASCs移植组、氯化锂移植组、氯化锂联合AASCs组和DMEM移植组.术后行体感诱发电位(SEP),运动诱发电位(MEP)检查和BBB评分.12周后进行10%BDA神经顺行示踪标记,切片后,行Cy3荧光探针染色、免疫组织化学及苏木素-伊红(HE)染色.结果术后4周BBB评分各组之间差异开始有统计学意义(F=46.897,P<0.05).实验结束时,SEP、MEP波幅值各组间差异有统计学意义(F-SEP=15.47,P<0.05;F-MEP=8.974,P<0.05);BDA示踪,HE和免疫组织化学染色各组间差异有统计学意义(P<0.05).结论氯化锂联合AASCs移植可以显著改善SCI后的功能恢复.
목적 관찰록화리연합이식자체격활설왕세포(AASCs)재치료척수손상(SCI)중적작용.방법 Wistar성서48지건립T10급성SCI모형.수궤균분위4조:AASCs이식조、록화리이식조、록화리연합AASCs조화DMEM이식조.술후행체감유발전위(SEP),운동유발전위(MEP)검사화BBB평분.12주후진행10%BDA신경순행시종표기,절편후,행Cy3형광탐침염색、면역조직화학급소목소-이홍(HE)염색.결과 술후4주BBB평분각조지간차이개시유통계학의의(F=46.897,P<0.05).실험결속시,SEP、MEP파폭치각조간차이유통계학의의(F-SEP=15.47,P<0.05;F-MEP=8.974,P<0.05);BDA시종,HE화면역조직화학염색각조간차이유통계학의의(P<0.05).결론 록화리연합AASCs이식가이현저개선SCI후적공능회복.
Objective To discuss the curative effectivenss of lithium chloride in combined with autologus activated Schwann cells (AASCs) in the treatment of spinal cord injury (SCI) in rats. Methods SCI models in 48 adult Wistar rats were made at T10 and divided into 4 groups:AASCs group,lithium chloride group, AASCs + lithium chloride group and DMEM group. After surgical operation, SEP, MEP and BBB score were made in every week. 10% BDA anterograde tracing mark, Cy3 fluorescence staining, HE staining and immunohistochemistry staining were made 12 months later. Results On the 4th week after grafting, there was statistically significant difference in BBB score between groups (ANOVA, F = 46.897, P < 0.05). At the end of the experiment, there was statistically significant difference in in SEP and MEP amplitudes of wave between groups (F-SEP = 15.47, P < 0.05 ; F-MEP = 8. 974, P < 0.05). Statistically significant difference was found in BDA tracing, HE and immunohistochemistry staining (P < 0.05). Con-clusion Evident amelioration after SCI can be found by co-transplanting AASCs and lithium chloride.