CAJ | 학술논문

目的建STAT3的RNAi载体干扰STAT3的表达，在体内外研究对胶质瘤干细胞的影响。方法构建STAT3基因shRNA的腺病毒表达载体，感染人胶质瘤干细胞。采用RT -PCR、Western blot检测STAT3基因的干扰效果，用MTT和流式细胞仪检测干细胞的增殖、凋亡，并观察对荷瘤裸鼠的影响。结果转染重组腺病毒Ad - STAT3 siRNA后，胶质瘤干细胞的STAT3的mRNA表达率为(41.5±7.3)％，蛋白表达率为（31.2±6.4）％，细胞的增殖受到抑制，凋亡率为(23.8±6.1)％，同时荷瘤裸鼠的肿瘤生长受到抑制，生存期延长。结论重组腺病毒介导的STAT3 RNAi可显著抑制靶基因的表达与活化，显著抑制胶质瘤干细胞的生长功能，为基于胶质瘤干细胞的功能治疗提供了基础理论。
목적 건STAT3적RNAi재체간우STAT3적표체，재체내외연구대효질류간세포적영향。방법 구건STAT3기인shRNA적선병독표체재체，감염인효질류간세포。채용RT -PCR、Western blot검측STAT3기인적간우효과，용MTT화류식세포의검측간세포적증식、조망，병관찰대하류라서적영향。결과전염중조선병독Ad - STAT3 siRNA후，효질류간세포적STAT3적mRNA표체솔위(41.5±7.3)％，단백표체솔위（31.2±6.4）％，세포적증식수도억제，조망솔위(23.8±6.1)％，동시하류라서적종류생장수도억제，생존기연장。결론중조선병독개도적STAT3 RNAi가현저억제파기인적표체여활화，현저억제효질류간세포적생장공능，위기우효질류간세포적공능치료제공료기출이론。
Objective To construct STAT3 siRNA to interfere the expression STAT3,and to observe the effect on glioblastoma stem cells. Method The adenovirus shRNA expression vector targeting STAT3 gene was constructed and transduced into glioblastoma stem cells. The gene silencing efficiency was monitored by RT - PCR and Western blot analysis. The proliferation and apoptosis of glioblastoma stem cells were detected by MTT assays and flow cytometry. In addition, the effect of tumor bearing mice was also observed. Results After the adenovirus STAT3 siRNA transfection, the level of mRNA decreased to (41.5 ±7.3)％, the level of protein decreased to( 31.2 ± 6. 4)％, the cell proliferation was inhibited, the cell apoptosis ratio increased to (23. 8 ± 6. 1 ) ％. Additionly, the adenovirus STAT3 siRNA could inhibit the tumor growth and improve the lifespan of mice. Conclusions The adenovirus shRNA targeting STAT3 gene could significantly inhibit the expression and activation of STAT3, and suppress the proliferation of glioblastoma stem cells, which provide an ideal therapeutic strategy for treatment of glioblastoma stem cells.