中华胸心血管外科杂志
中華胸心血管外科雜誌
중화흉심혈관외과잡지
Chinese Journal of Thoracic and Cardiovascular Surgery
2010年
1期
45-48
,共4页
邵明海%胡炜%王建华%卢洪胜%陈仕林
邵明海%鬍煒%王建華%盧洪勝%陳仕林
소명해%호위%왕건화%로홍성%진사림
胸腺肿瘤%癌%鳞状细胞%多态性%单核苷酸%微卫星不稳定%杂合子丢失
胸腺腫瘤%癌%鱗狀細胞%多態性%單覈苷痠%微衛星不穩定%雜閤子丟失
흉선종류%암%린상세포%다태성%단핵감산%미위성불은정%잡합자주실
Thymus neoplasms%Carcinoma squamous cell%Polymorphism%single nucleotide mierosateUite in-stability%Loss of heterozygosity
目的 检测胸腺鳞癌微卫星不稳定(MSI)和杂合性缺失(LOH)发生频率,以探讨胸腺鳞癌MSI现象的合适微卫星(MS)位点.方法 选择5个微卫星多态性标记,从石蜡包埋的存档标本中选取9例肿瘤组织和其对应的自身正常组织,提取DNA后用PCR扩增,6%聚丙稀酰胺凝胶电泳,银染显色后进行MSI和LOH分析.结果 9例胸腺鳞癌均出现MSI或LOH.在所检5个位点中D6S1708、TP53、DM、D11S988和D8S136微卫星不平衡发生率分别为66.7%(6/9)、33.3%(3/9)、33.3%(3/9)、33.3%(3/9)和0%(0/9).D6S1708遗传学改变多为LOH(5/6),D11S988位点仅见于LOH.结论 D6S1708、TP53、DM和D11S988可以作为研究胸腺鳞癌微卫星的位点;微卫星不平衡可能在胸腺鳞癌的发生中起一定作用,其与胸腺鳞癌临床病理特点的关系尚需进一步探讨.
目的 檢測胸腺鱗癌微衛星不穩定(MSI)和雜閤性缺失(LOH)髮生頻率,以探討胸腺鱗癌MSI現象的閤適微衛星(MS)位點.方法 選擇5箇微衛星多態性標記,從石蠟包埋的存檔標本中選取9例腫瘤組織和其對應的自身正常組織,提取DNA後用PCR擴增,6%聚丙稀酰胺凝膠電泳,銀染顯色後進行MSI和LOH分析.結果 9例胸腺鱗癌均齣現MSI或LOH.在所檢5箇位點中D6S1708、TP53、DM、D11S988和D8S136微衛星不平衡髮生率分彆為66.7%(6/9)、33.3%(3/9)、33.3%(3/9)、33.3%(3/9)和0%(0/9).D6S1708遺傳學改變多為LOH(5/6),D11S988位點僅見于LOH.結論 D6S1708、TP53、DM和D11S988可以作為研究胸腺鱗癌微衛星的位點;微衛星不平衡可能在胸腺鱗癌的髮生中起一定作用,其與胸腺鱗癌臨床病理特點的關繫尚需進一步探討.
목적 검측흉선린암미위성불은정(MSI)화잡합성결실(LOH)발생빈솔,이탐토흉선린암MSI현상적합괄미위성(MS)위점.방법 선택5개미위성다태성표기,종석사포매적존당표본중선취9례종류조직화기대응적자신정상조직,제취DNA후용PCR확증,6%취병희선알응효전영,은염현색후진행MSI화LOH분석.결과 9례흉선린암균출현MSI혹LOH.재소검5개위점중D6S1708、TP53、DM、D11S988화D8S136미위성불평형발생솔분별위66.7%(6/9)、33.3%(3/9)、33.3%(3/9)、33.3%(3/9)화0%(0/9).D6S1708유전학개변다위LOH(5/6),D11S988위점부견우LOH.결론 D6S1708、TP53、DM화D11S988가이작위연구흉선린암미위성적위점;미위성불평형가능재흉선린암적발생중기일정작용,기여흉선린암림상병리특점적관계상수진일보탐토.
Objective To investigate the frequency of micresatellite instability (MSI) and loss of heterozygosity (LOH) and select sensitive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Methods 5 microsat-ellitc polymorphism markers and extrated DNA were selected from 9 specmiens of paired thymie squamous cell carcinoma/nor-real tissues. MSI and LOH in the specmiens of thymic carcinoma and relevant pericancerou tissues were detected by polymerase chain reaction (PCR) followed by 6% polyacrylamide gel electrophoresis(PAGE) with silver staining. Results MSI or LOH was detected in 9 thymic carcinoma tissues. The frequency of MSI or LOH was 66.7% (6/9) at loci of D6S1708, 33.3% (3/9) at TP53, 33.3% (3/9)at DM, 33.3% (3/9)at D11S988 and 0% (0/9)at D8S136, LOH at D6S1708 (5/6) was a common genetic alteration. DI1S988 had only LOH alteration. Conclusion D6S1708, TP53, DM, and D11S988 are sensi-tive loci for studying microsatellite DNA imbalance in thymic squamous cell carcinoma. Microsatellite DNA imbalance may play a certain role in occurrence and development of thymic squamous cell carcinoma, and the relationship between MSi or LOH.The linicopathological features of thymic squamous cell carcinoma needs further investigation.
