中华外科杂志
中華外科雜誌
중화외과잡지
CHINESE JOURNAL OF SURGERY
2010年
17期
1309-1312
,共4页
张海宁%冷萍%王英振%吕成昱%王湘达%王昌耀
張海寧%冷萍%王英振%呂成昱%王湘達%王昌耀
장해저%랭평%왕영진%려성욱%왕상체%왕창요
组织工程%半月板,胫骨%缺损
組織工程%半月闆,脛骨%缺損
조직공정%반월판,경골%결손
Tissue engineering%Menisci,tibial%Defect
目的 观察骨髓基质干细胞复合藻酸钙凝胶注射式修复全层半月板无血运区缺损的效果.方法 2008年6月至2009年2月制造成年山羊半月板前角无血运区全层缺损模型.以自体骨髓基质干细胞复合可注射藻酸钙凝胶修复半月板缺损(Ⅰ组),同时设立单纯载体组(Ⅱ组)和空白对照组(Ⅲ组).术后4、8、16周处死动物,行大体观察,组织学观察、电镜观察和MRI检查并比较修复效果.结果 Ⅰ组缺损完全被修复组织填充,结合紧密,与正常半月板组织相似,在4~16周效果逐渐改善,大体观察优于其他各组.光镜见细胞随凝胶纤维排列分布,载体纤维间隙大多为细胞分泌基质所充填,细胞排列密集,基质分布均匀.透射电镜见Ⅰ组细胞呈软骨细胞样形态,细胞突起较多,细胞器丰富,细胞为不同走向排列的纤维包绕.MRI检查发现Ⅰ组修复效果较好.结论 骨髓基质干细胞复合藻酸钙凝胶注射可有效地修复全层半月板无血运区缺损.
目的 觀察骨髓基質榦細胞複閤藻痠鈣凝膠註射式脩複全層半月闆無血運區缺損的效果.方法 2008年6月至2009年2月製造成年山羊半月闆前角無血運區全層缺損模型.以自體骨髓基質榦細胞複閤可註射藻痠鈣凝膠脩複半月闆缺損(Ⅰ組),同時設立單純載體組(Ⅱ組)和空白對照組(Ⅲ組).術後4、8、16週處死動物,行大體觀察,組織學觀察、電鏡觀察和MRI檢查併比較脩複效果.結果 Ⅰ組缺損完全被脩複組織填充,結閤緊密,與正常半月闆組織相似,在4~16週效果逐漸改善,大體觀察優于其他各組.光鏡見細胞隨凝膠纖維排列分佈,載體纖維間隙大多為細胞分泌基質所充填,細胞排列密集,基質分佈均勻.透射電鏡見Ⅰ組細胞呈軟骨細胞樣形態,細胞突起較多,細胞器豐富,細胞為不同走嚮排列的纖維包繞.MRI檢查髮現Ⅰ組脩複效果較好.結論 骨髓基質榦細胞複閤藻痠鈣凝膠註射可有效地脩複全層半月闆無血運區缺損.
목적 관찰골수기질간세포복합조산개응효주사식수복전층반월판무혈운구결손적효과.방법 2008년6월지2009년2월제조성년산양반월판전각무혈운구전층결손모형.이자체골수기질간세포복합가주사조산개응효수복반월판결손(Ⅰ조),동시설립단순재체조(Ⅱ조)화공백대조조(Ⅲ조).술후4、8、16주처사동물,행대체관찰,조직학관찰、전경관찰화MRI검사병비교수복효과.결과 Ⅰ조결손완전피수복조직전충,결합긴밀,여정상반월판조직상사,재4~16주효과축점개선,대체관찰우우기타각조.광경견세포수응효섬유배렬분포,재체섬유간극대다위세포분비기질소충전,세포배렬밀집,기질분포균균.투사전경견Ⅰ조세포정연골세포양형태,세포돌기교다,세포기봉부,세포위불동주향배렬적섬유포요.MRI검사발현Ⅰ조수복효과교호.결론 골수기질간세포복합조산개응효주사가유효지수복전층반월판무혈운구결손.
Objective To investigate the effectiveness of injectable tissue engineering to repair fullthickness meniscal defects. Methods From June 2008 to February 2009 full-thickness of meniscal defects were created in the anterior corner of goats, which with no blood supply, in a diameter of 2 mm. Then bone marrostem cells(BMSCs) was mixed with injectable calcium alginate gel to fill the defects. Other groups include the calcium alginate gel and empty group were served as control groups. At different time points, the animals were sacrificed and macroscopy, microscopy determination, electroscopy and MRI detection were performed to assess the outcomes of repairment. Results The meniscal defects had been filled thoroughly in 16 weeks after operation with white,tough and elastic repair tissue similar to normal meniscal fibrocartilage in the tissue engineering groups. The repair tissue was mainly fibrochondrocytes in line with the calcium alginate fiber. Thick matrix secreted by the cells crammed the space between fibers. The view under electroscopy demonstrated that the microstructure of the repair tissue was normal and cells were in a fibrocartilage phenotype. Conclusion The full-thickness meniscal defects in regions without blood supply can be reconstructed effectively with injectable tissue engineering.